Logo image
Back
Failure of a recombinant Babesia bovis antigen to protect cattle against heterologous strain challenge
Journal article   Peer reviewed

Failure of a recombinant Babesia bovis antigen to protect cattle against heterologous strain challenge

Peter Timms, D N Barry, A C Gill, P J Sharp and A J de Vos
Research in Veterinary Science, Vol.45(2), pp.267-269
1988
DOI: https://doi.org/10.1016/S0034-5288(18)30947-0
url
https://doi.org/10.1016/S0034-5288(18)30947-0View
Published Version

Abstract

Groups of cattle were inoculated subcutaneously with (i) a recombinant DNA-derived Babesia bovis protein (KaBbl-GZ) fused to beta-galactosidase and combined with adjuvants, or (ii) native beta-galactosidase (GZ) plus adjuvant, or (iii) adjuvant only or (iv) a live, attenuated B bovis vaccine. KaBbl-GZ was produced in the lambda gt11-amp3 system as a 5-10 kD babesial polypeptide linked to GZ. KaBbl has previously been shown to be an immunodominant antigen of B bovis, localised at the apex of the parasite, and present in a range of B bovis strains. High levels of GZ antibodies were observed in KaBbl-GZ and GZ inoculated cattle, but specific KaBbl antibodies could not be detected by ELISA. Five months after primary inoculation, all cattle were blood challenged with a virulent heterologous B bovis strain. Despite four inoculations with KaBbl-GZ, significant protection against the challenge was not observed.

Details

Metrics

548 Record Views
12 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types
Domestic collaboration
Web Of Science research areas
Veterinary Sciences

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

Source: InCites

Logo image