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Transfusion - 2024 - Hwang - Extracellular vesicles in fresh frozen plasma and cryoprecipitate Impact on in vitro1.45 MB
Published VersionCC BY-NC-ND V4.0, Open Access
Journal article
Extracellular vesicles in fresh frozen plasma and cryoprecipitate: Impact on in vitro endothelial cell viability
Transfusion , Vol.64(9), pp.1709-1718
2024
Abstract
Background
Transfusion-related acute lung injury (TRALI) remains a major contributor to transfusion-associated mortality. While the pathogenesis of TRALI remains unclear, there is evidence of a role for blood components. We therefore investigated the potential effects of fresh frozen plasma (FFP), cryoprecipitate, and extracellular vesicles (EVs) derived from these blood components, on the viability of human lung microvascular endothelial cells (HLMVECs) in vitro.
Methods
EVs were isolated from FFP and cryoprecipitate using size-exclusion chromatography and characterized by nanoparticle tracking analysis, western blotting, and transmission electron microscopy. The potential effects of these blood components and their EVs on HLMVEC viability (determined by trypan blue exclusion) were examined in the presence and absence of neutrophils, either with or without prior treatment of HLMVECs with LPS.
Results
EVs isolated from FFP and cryoprecipitate displayed morphological and biochemical properties conforming to latest international criteria. While FFP, cryoprecipitate, and EVs derived from FFP, each reduced HLMVEC viability, no effect was observed for EVs derived from cryoprecipitate.
Conclusion
Our findings demonstrate clear differences in the effects of FFP, cryoprecipitate, and their respective EVs on HLMVEC viability in vitro. Examination of the mechanisms underlying these differences may lead to an improved understanding of the factors that promote development of TRALI.
Details
- Title
- Extracellular vesicles in fresh frozen plasma and cryoprecipitate: Impact on in vitro endothelial cell viability
- Authors
- Ji Hui Hwang (Corresponding Author) - Queensland University of TechnologyJohn-Paul Tung - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - LegacyDamien G. Harkin - School of Biomedical Sciences, Faculty of Health Queensland University of Technology Brisbane Queensland Australia, Strategy and Growth, Australian Red Cross Lifeblood Brisbane Queensland AustraliaRobert L. Flower - Australian Red Cross LifebloodNatalie M. Pecheniuk - School of Biomedical Sciences, Faculty of Health Queensland University of Technology Brisbane Queensland Australia, Strategy and Growth, Australian Red Cross Lifeblood Brisbane Queensland Australia
- Publication details
- Transfusion , Vol.64(9), pp.1709-1718
- Publisher
- Wiley-Blackwell Publishing, Inc.
- Date published
- 2024
- DOI
- 10.1111/trf.17959
- ISSN
- 1537-2995; 0041-1132
- Copyright note
- © 2024 The Author(s). Transfusion published by Wiley Periodicals LLC on behalf of AABB. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
- Organisation Unit
- School of Health; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 991051598602621
- Output Type
- Journal article
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- Hematology
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