Journal article
External quality assessment of human neutrophil antigen (HNA)-specific antibody detection and HNA genotyping from 2000 to 2012
Vox Sanguinis, Vol.105(3), pp.259-269
2013
Abstract
Since 2000, Quality Assurance (QA) exercises for the detection and identification of granulocyte antibodies and DNA typing for human neutrophil antigens (HNA) have been distributed within the International Granulocyte Immunobiology Workshops, which are linked to International Society of Blood Transfusion. The exercises were standardised at the outset to enable laboratory performance to be monitored. Between 2000 and 2012, nine exercises were distributed to 20 laboratories. Overall, 45 examples of 42 unique samples containing defined granulocyte reactive antibodies were distributed for serological analysis together with 20 samples for HNA genotyping. The level of satisfactory serological performance was initially set at 50% and later increased to 70%, while the 'cut-off' for HNA genotyping was set at 100% after 2008. Failure to achieve the minimum score in the QA exercises in consecutive years resulted in temporary exclusion. In 2000, the 15 participating laboratories had a mean score of 56.1% for serological analysis and 13 laboratories attempted HNA-1a and -1b genotyping, while 11 attempted HNA-1c typing. Steady improvements in proficiency for serological testing and HNA typing occurred in subsequent exercises. In 2012, the mean score for serology was 88.5% and 12/13 laboratories scored 100% for HNA-1a, -1b, -1c, -3a, -3b, -4a, -4bw, -5a and -5bw genotyping. These QA exercises have provided an invaluable tool to monitor and improve the standard of granulocyte immunology investigations for participating laboratories, thereby enhancing performance for both clinical investigations and donor screening programmes to reduce the incidence of TRALI.
Details
- Title
- External quality assessment of human neutrophil antigen (HNA)-specific antibody detection and HNA genotyping from 2000 to 2012
- Authors
- G Lucas (Author) - NHS Blood and Transplant, United KingdomL Porcelijn (Author) - Sanquin Diagnostic Services, NetherlandsYoke Lin Fung (Author) - University of QueenslandF Green (Author) - NHS Blood and Transplant, United KingdomA Reil (Author) - DRK Blood Service West, GermanyM Hopkins (Author) - NHS Blood and Transplant, United KingdomR Schuller (Author) - American Red Cross, United StatesA Green (Author) - NHS Blood and Transplant, United KingdomM de Haas (Author) - Sanquin Diagnostic Services, NetherlandsJ Bux (Author) - DKR Blood Service West, Germany
- Publication details
- Vox Sanguinis, Vol.105(3), pp.259-269
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2013
- DOI
- 10.1111/vox.12041
- ISSN
- 0042-9007
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449064902621
- Output Type
- Journal article
Metrics
1 File views/ downloads
711 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Hematology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites