Logo image
Back
Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis
Journal article   Peer reviewed

Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis

S M Prabhu, M L Meistrich, E A McLaughlin, S D Roman, S Warne, S Mendis, Catherine Itman and K L Loveland
Reproduction, Vol.131(3), pp.489-499
2006
DOI: https://doi.org/10.1530/rep.1.00968
url
https://doi.org/10.1530/rep.1.00968View
Published Version

Abstract

gonadorelin antagonist messenger RNA stem cell factor receptor
Germ cell proliferation, migration and survival during all stages of spermatogenesis are affected by stem cell factor signalling through the c-Kit receptor, the expression and function of which are vital for normal male reproductive function. The present study comprehensively describes the c-Kit mRNA and protein cellular expression profiles in germ cells of the postnatal and adult rodent testis, revealing their significant elevation in synthesis at the onset of spermatogenesis. Real-time PCR analysis for both mice and rats matched the cellular mRNA expression profile where examined. Localization studies in normal mouse testes indicated that both c-Kit mRNA and protein are first detectable in differentiating spermatogonia. In addition, all spermatogonia isolated from 8-day-old mice displayed detectable c-Kit mRNA, but 30-50% of these lacked protein expression. The c-Kit mRNA and protein profile in normal rat testes indicated expression in gonocytes, in addition to differentiating spermatogonia. However, in the irradiated adult rat testes, in which undifferentiated spermatogonia are the only germ cell type, mRNA was also detected in the absence of protein. This persisted at 3 days and 1 and 2 weeks following treatment with gonadotrophin-releasing hormone (GnRH) antagonist to stimulate spermatogenesis recovery. By 4 weeks of GnRH antagonist treatment, accompanying the emergence of differentiating spermatogonia, both mRNA and protein were detected. Based on these observations, we propose that c-Kit mRNA and protein synthesis are regulated separately, possibly by influences linked to testis maturation and circulating hormone levels. © 2006 Society for Reproduction and Fertility.

Details

Metrics

369 Record Views
65 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types
Domestic collaboration
International collaboration
Web Of Science research areas
Developmental Biology
Reproductive Biology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

Source: InCites

Logo image