Expression of an Androgenic-Gland-Specific Insulin-Like Peptide during the Course of Prawn Sexual and Morphotypic Differentiation

Tomer Ventura; R Manor; E D Aflalo; S Weil; I Khalaila; O Rosen; A Sagi

doi:10.5402/2011/476283

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Expression of an Androgenic-Gland-Specific Insulin-Like Peptide during the Course of Prawn Sexual and Morphotypic Differentiation

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Expression of an Androgenic-Gland-Specific Insulin-Like Peptide during the Course of Prawn Sexual and Morphotypic Differentiation

Tomer Ventura, R Manor, E D Aflalo, S Weil, I Khalaila, O Rosen and A Sagi

ISRN Endocrinology, Vol.2011, pp.1-11

2011

DOI: https://doi.org/10.5402/2011/476283

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Abstract

Genetics

Evolutionary Biology

The crustacean male-specific androgenic gland (AG) regulates sexual differentiation. In the prawn Macrobrachium rosenbergii, silencing an AG-specific insulin-like encoding transcript (Mr-IAG) inhibited the development of male sexual characters, suggesting that Mr-IAG is a key androgenic hormone. We used recombinant pro-Mr-IAG peptide to generate antibodies that recognized the peptide in AG cells and extracts, as verified by mass spectrometry. We revealed the temporal expression pattern of Mr-IAG and studied its relevance to the timetable of sex differentiation processes in juveniles and after puberty. Mr-IAG was expressed from as early as 20 days after metamorphosis, prior to the appearance of external male sexual characters. Mr-IAG expression was lower in the less reproductively active orange-clawed males than in both the dominant blue-clawed males and the actively sneak mating small males. These results suggest a role for Mr-IAG both in the timing of male sexual differentiation and in regulating reproductive strategies.

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Title: Expression of an Androgenic-Gland-Specific Insulin-Like Peptide during the Course of Prawn Sexual and Morphotypic Differentiation
Authors: Tomer Ventura (Author) - Ben-Gurion University of the Negev, Israel
R Manor (Author) - Ben-Gurion University of the Negev, Israel
E D Aflalo (Author) - Ben-Gurion University of the Negev, Israel
S Weil (Author) - Ben-Gurion University of the Negev, Israel
I Khalaila (Author) - Ben-Gurion University of the Negev, Israel
O Rosen (Author) - Ben-Gurion University of the Negev, Israel
A Sagi (Author) - Ben-Gurion University of the Negev, Israel
Publication details: ISRN Endocrinology, Vol.2011, pp.1-11
Publisher: Hindawi Publishing Corporation
Date published: 2011
DOI: 10.5402/2011/476283
ISSN: 2090-4630
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450371402621
Output Type: Journal article

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