Expression library immunization confers partial protection against Chlamydia muridarum genital infection

C L McNeilly; K W Beagley; R J Moore; V Haring; Peter Timms; L M Hafner

doi:10.1016/j.vaccine.2006.12.019

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Expression library immunization confers partial protection against Chlamydia muridarum genital infection

Journal article

Peer reviewed

Expression library immunization confers partial protection against Chlamydia muridarum genital infection

C L McNeilly, K W Beagley, R J Moore, V Haring, Peter Timms and L M Hafner

Vaccine, Vol.25(14), pp.2643-2655

2007

DOI: https://doi.org/10.1016/j.vaccine.2006.12.019

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Abstract

chlamydia

vaccines

expression library immunization

gene gun

Protective sequences of Chlamydia muridarum were identified as potential vaccine candidates by screening a genomic DNA expression library and assessing the immune responses of mice immunized with individual library clones following vaginal challenge with live Chlamydia. Groups of female BALB/c mice were immunized intra-abdominally by gene gun delivery of DNA three times at three-weekly intervals with individual library clones expressing chlamydial protein fragments and humoral and cell-mediated immune responses were evaluated. Chlamydia-specific cytokines including tumour necrosis factor-? (TNF-?) interleukin-10 (IL-10), interleukin-4 (IL-4), interleukin-12 (IL-12) and interferon-? (IFN-?) were detected in mice immunized either with selected DNA clones in spleen cells (0.2-135.2 pg/mL) or lymph nodes (0.15-84.9 pg/mL). The most protective antigen identified was TC0512, a putative outer membrane protein (OMP). Immunization of mice with this clone elicited T-helper type-1 (Th-1) and T-helper type-2 (Th-2) cytokines as well as and IgG1 and IgG2a in sera of these animals. Ten days after the last immunization, animals were challenged intra-vaginally with 5 × 104 inclusion-forming units (IFUs) of C. muridarum. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls. Six additional clones were identified that also conferred varying degrees of protection against live chlamydial challenge. Significant protection against the initial stages of infection was shown by two DNA clones (encoding hypothetical proteins) and five clones showed enhanced clearance of chlamydial infection following DNA immunization and live chlamydial challenge. These results demonstrate that the C. muridarum genome can be screened for individual vaccine candidates by genetic immunization and that the screen produces novel and partially protective vaccine candidates. © 2007 Elsevier Ltd. All rights reserved.

Details

Title: Expression library immunization confers partial protection against Chlamydia muridarum genital infection
Authors: C L McNeilly (Author) - University of Surrey, United Kingdom
K W Beagley (Author) - University of Newcastle
R J Moore (Author) - CSIRO Livestock Industries
V Haring (Author) - CSIRO Livestock Industries
Peter Timms (Author) - Queensland University of Technology
L M Hafner (Author) - Queensland University of Technology
Publication details: Vaccine, Vol.25(14), pp.2643-2655
Publisher: Elsevier Ltd.
Date published: 2007
DOI: 10.1016/j.vaccine.2006.12.019
ISSN: 0264-410X
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449876302621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Immunology; Medicine, Research & Experimental

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