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Accepted VersionCC BY-NC-ND V4.0, Open Access
Journal article
Expression and methylation of BDNF in the human brain in schizophrenia
World Journal of Biological Psychiatry, Vol.18(5), pp.392-400
2017
PMID: 27712141
Abstract
Objectives: To examine the combined effect of the BDNF Val66Met (rs6265) polymorphism and BDNF DNA methylation on transcriptional regulation of the BDNF gene.
Methods: DNA methylation profiles were generated for CpG sites proximal to Val66Met, within BDNF promoter I and exon V for prefrontal cortex samples from 25 schizophrenia and 25 control subjects. Val66Met genotypes and BDNF mRNA expression data were generated by transcriptome sequencing. Expression, methylation and genotype data were correlated and examined for association with schizophrenia.
Results: There was 43% more of the BDNF V-VIII-IX transcript in schizophrenia samples. BDNF mRNA expression and DNA methylation of seven CpG sites were not associated with schizophrenia after accounting for age and PMI effects. BDNF mRNA expression and DNA methylation were not altered by Val66Met after accounting for age and PMI effects. DNA methylation of one CpG site had a marginally significant positive correlation with mRNA expression in schizophrenia subjects.
Conclusions: Schizophrenia risk was not associated with differential BDNF mRNA expression and DNA methylation. A larger age-matched cohort with comprehensive clinical history is required to accurately identify the effects of genotype, mRNA expression and DNA methylation on schizophrenia risk.
Details
- Title
- Expression and methylation of BDNF in the human brain in schizophrenia
- Authors
- Sern-Yih Cheah (Author) - The University of QueenslandRobert McLeay (Author) - Queensland University of TechnologyLeesa F Wockner (Author) - QIMR Berghofer Medical Research InstituteBruce R Lawford (Author) - Queensland University of TechnologyRoss Young (Author) - Queensland University of TechnologyCharles P Morris (Author) - Queensland University of TechnologyJoanne Voisey (Author) - Queensland University of Technology
- Publication details
- World Journal of Biological Psychiatry, Vol.18(5), pp.392-400
- Publisher
- Taylor & Francis
- Date published
- 2017
- DOI
- 10.1080/15622975.2016.1245443
- ISSN
- 1814-1412; 1562-2975
- PMID
- 27712141
- Copyright note
- Copyright (c) 2017. This is an Accepted Manuscript of an article published by Taylor & Francis in World Journal of Biological Psychiatry on 2017, available online: http://www.tandfonline.com/10.1080/15622975.2016.1245443
- Organisation Unit
- University of the Sunshine Coast, Queensland; Office of the Deputy Vice-Chancellor (Research and Innovation)
- Language
- English
- Record Identifier
- 99550997102621
- Output Type
- Journal article
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