Journal article
Expression and localization of thioredoxin during early implantation in the marmoset monkey
Molecular Human Reproduction, Vol.7(12), pp.1159-1165
2001
PMID: 11719593
Abstract
Thioredoxin is a powerful redox protein expressed in invasive cytotrophoblasts and essential for blastocyst implantation in mice. Isolated marmoset thioredoxin cDNA showed that the deduced amino acid sequence differed from the human sequence by four amino acids. The close homology of thioredoxin in the two species enabled us to use monoclonal antibodies against human thioredoxin to detect marmoset thioredoxin in implantation sites, blastocysts and culture medium. Immunocytochemistry on marmoset implantation sites, on pregnancy days 12 and 15, showed that thioredoxin is highly expressed in uterine luminal epithelium, glands and in some endometrial stromal cells. In attached blastocysts, thioredoxin staining was detected in mural and polar trophoblast cells and both visceral and parietal endoderm, whereas no staining was present in the inner cell mass. A similar pattern of thioredoxin expression was detected in hatched blastocysts attached to Matrigel in tissue culture. Trophoblastic vesicles derived from blastocysts expressed thioredoxin in inner endoderm-like cells and outer trophoblast-like cells and secreted thioredoxin into the culture medium. These experiments have demonstrated thioredoxin expression during early stages of embryo–maternal interaction. We propose that thioredoxin protects the early placenta from oxidative damage and that the marmoset is a valuable model for studying thioredoxin regulation and function during implantation and blastocyst differentiation.
Details
- Title
- Expression and localization of thioredoxin during early implantation in the marmoset monkey
- Authors
- A. Lopata (Author) - University of MelbourneM.C. Sibson (Author) - University of MelbourneA.C. Enders (Author) - University of California, DavisK.L. Bloomfield (Author) - Griffith UniversityM.S. Gregory - Griffith UniversityG.Di Trapani (Author) - Griffith UniversityA.V. Perkins (Author) - Griffith UniversityK.F. Tonissen (Corresponding Author) - University of California, DavisF.M. Clarke (Author) - Griffith University
- Publication details
- Molecular Human Reproduction, Vol.7(12), pp.1159-1165
- Publisher
- Oxford University Press
- DOI
- 10.1093/molehr/7.12.1159
- ISSN
- 1460-2407
- PMID
- 11719593
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy; School of Health
- Language
- English
- Record Identifier
- 99685197502621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Developmental Biology
- Obstetrics & Gynecology
- Reproductive Biology
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Source: InCites