Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the sydney rock oyster

M N Andrew; Wayne A O'Connor; R H Dunstan; G R MacFarlane

doi:10.1007/s10646-010-0529-5

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Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the sydney rock oyster

Journal article

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Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the sydney rock oyster

M N Andrew, Wayne A O'Connor, R H Dunstan and G R MacFarlane

Ecotoxicology, Vol.19(8), pp.1440-1451

2010

DOI: https://doi.org/10.1007/s10646-010-0529-5

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Abstract

17α, biomarker

estrogens

ethynylestradiol

intersex

oyster

vitellogenin

ethinylestradiol

biological marker

estrogen

bioaccumulation

developmental stage

dose-response relationship

ecotoxicology

estrogenic compound

mollusc

pollution effect

Although mounting evidence suggests exposure to estrogenic contaminants increases vitellogenin production in molluscs, demonstration of dose-response relationships and knowledge of the temporal nature of the vitellogenin response with continual exposure is currently lacking for biomarker utility. To address this knowledge gap, adult Sydney rock oysters, Saccostrea glomerata, were exposed to a range of environmentally relevant concentrations of 17α-ethynylestradiol (EE2) (0, 6.25, 12.5, 25 or 50 ng/l) in seawater under laboratory conditions. Vitellogenin induction and gonadal development was assessed following 4, 21 and 49 days exposure to EE2. Vitellogenin was found to increase in a dose dependent manner with EE2 exposure for females (4 and 49 days) and males (4 and 21 days). Histological examination of gonads revealed a number of individuals exhibited intersex (ovotestis) in 50 ng/l EE2 (after 21 days) and in 6.25 and 12.5 ng/l EE2 (after 49 days). Furthermore, a significant shift towards females was observed following 49 days exposure at 50 ng/l EE2 suggesting estrogenic exposure is capable of facilitating a progression for protandric males from male-intersex-female gametal status. Increases in female vitellogenin (4 days) were predictive of later increases in female developmental stages at 21 days and increases in oocyte area following 49 days. Male vitellogenin (4 days) was predictive of decreased male percentages and lower male developmental stages at 49 days. Vitellogenin in S. glomerata is a predictive biomarker of estrogenic exposure and effect if sampled soon after exposure and at the commencement of a gonadal development cycle. © Springer Science+Business Media, LLC 2010.

Details

Title: Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the sydney rock oyster
Authors: M N Andrew (Author) - University of Newcastle
Wayne A O'Connor (Author) - Port Stephens Fisheries Centre
R H Dunstan (Author) - University of Newcastle
G R MacFarlane (Author) - University of Newcastle
Publication details: Ecotoxicology, Vol.19(8), pp.1440-1451
Publisher: Springer New York LLC
Date published: 2010
DOI: 10.1007/s10646-010-0529-5
ISSN: 0963-9292
Organisation Unit: University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
Language: English
Record Identifier: 99451030502621
Output Type: Journal article

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