Existence of an egg-laying hormone-like peptide in male reproductive system of the giant freshwater prawn, Macrobrachium rosenbergii

Thanapong Kruangkum; Jirawat Saetan; Charoonroj Chotwiwatthanakun; Rapeepun Vanichviriyakit; Scott F Cummins; Chatip Wanichanon; Prasert Sobhon

doi:10.1016/j.acthis.2018.11.007

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Existence of an egg-laying hormone-like peptide in male reproductive system of the giant freshwater prawn, Macrobrachium rosenbergii

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Existence of an egg-laying hormone-like peptide in male reproductive system of the giant freshwater prawn, Macrobrachium rosenbergii

Thanapong Kruangkum, Jirawat Saetan, Charoonroj Chotwiwatthanakun, Rapeepun Vanichviriyakit, Scott F Cummins, Chatip Wanichanon and Prasert Sobhon

Acta Histochemica, Vol.121(2), pp.156-163

2019

DOI: https://doi.org/10.1016/j.acthis.2018.11.007

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Abstract

ELH-like peptide

male reproductive system

macrobrachium rosenbergii

The giant freshwater prawn,Macrobrachium rosenbergii, is an important aquaculture species. A better under-standing of the molecular components of reproduction in this species would help to advance the prawn pro-duction. In the present study, we demonstrated the presence of an egg laying hormone (ELH)-like peptide in themale reproductive system. First, an antibody to the abalone (a)ELH was generated, and by Western blot it wasshown to specifically bound to a protein from the maleM. rosenbergiireproductive tissues with a similar size tomolluscan ELH. This aELH-like peptide was localized in spermatogonia in the testes of all three male morpho-types: blue claw, orange claw and small males. Moreover, the aELH-like peptide was detected in the epitheliumof the spermatic duct and its associated smooth muscle cell layers and on the outer surface of spermatozoa. Aswell, the aELH-like peptide was detected in the spermatophore located in the female thelycum at 4-6 h post-mating, indicating that it was transferred to the female during copulation. Taken together, we suggest that thisaELH-like peptide could be as a male inducing factor that helped to accelerate female spawning. Liquid chro-matography of crude extracts and immunoblot analysis suggested that the aELH-like peptide could be furtherpurified for ultimate characterization.

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Title: Existence of an egg-laying hormone-like peptide in male reproductive system of the giant freshwater prawn, Macrobrachium rosenbergii
Authors: Thanapong Kruangkum (Author) - Mahidol University, Thailand
Jirawat Saetan (Author) - Prince of Songkla University, Thailand
Charoonroj Chotwiwatthanakun (Author) - Mahidol University, Thailand
Rapeepun Vanichviriyakit (Author) - Mahidol University, Thailand
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Chatip Wanichanon (Author) - Mahidol University, Thailand
Prasert Sobhon (Author) - Mahidol University, Thailand
Publication details: Acta Histochemica, Vol.121(2), pp.156-163
Publisher: Elsevier GmbH - Urban und Fischer
Date published: 2019
DOI: 10.1016/j.acthis.2018.11.007
ISSN: 0065-1281
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450857002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Cell Biology