Journal article
Excited state complex formation between 3-aminophthalhydrazide and DNA: a fluorescence quenching reaction
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol.55(13), pp.2711-2717
1999
Abstract
Fluorescence quenching reaction of 3-aminophthalhyrdazide (luminol) due to the interaction with short DNA fragments has been studied employing steady-state and time correlated single photon counting techniques. The interaction of luminol with DNA in the excited state has also been analysed using Stern–Volmer (S–V) mechanism. A very weak ground state interaction is proposed to explain the higher values of the quenching rate constant and is consistent with positive curvatures in the S–V plots. A plausible explanation of the quenching mechanism has been discussed on the basis of hydrogen bonding interaction between luminol and DNA fragment. It is proposed that the phosphate backbone present in DNA is responsible for the interaction with luminol.
Details
- Title
- Excited state complex formation between 3-aminophthalhydrazide and DNA: a fluorescence quenching reaction
- Authors
- G Will (Author) - University College DublinE Kudryashov (Author) - University College DublinE Duggan (Author) - University College DublinD Fitzmaurice (Author) - University College DublinV Buckin (Author) - University College DublinE Waghorne (Author) - University College DublinS Mukherjee (Corresponding Author) - University College Dublin
- Publication details
- Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol.55(13), pp.2711-2717
- Publisher
- Elsevier BV
- DOI
- 10.1016/S1386-1425(99)00139-0
- ISSN
- 1873-3557
- Organisation Unit
- School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99737876002621
- Output Type
- Journal article
Metrics
2 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Spectroscopy
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites