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Published VersionCC BY V4.0, Open Access
Journal article
Evaluation of three rapid low-resource molecular tests for Nipah virus
Frontiers in Microbiology, Vol.13, pp.1-12
2023
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Abstract
Accurate and timely diagnosis of Nipah virus (NiV) requires rapid, inexpensive, and robust diagnostic tests to control spread of disease. Current state of the art technologies are slow and require laboratory infrastructure that may not be available in all endemic settings. Here we report the development and comparison of three rapid NiV molecular diagnostic tests based on reverse transcription recombinase-based isothermal amplification coupled with lateral flow detection. These tests include a simple and fast one-step sample processing step that inactivates the BSL-4 pathogen, enabling safe testing without the need for multi-step RNA purification. The rapid NiV tests targeted the Nucleocapsid protein (N) gene with analytical sensitivity down to 1,000 copies/μL for synthetic NiV RNA and did not cross-react with RNA of other flaviviruses or Chikungunya virus, which can clinically present with similar febrile symptoms. Two tests detected 50,000–100,000 TCID50/mL (100–200 RNA copies/reaction) of the two distinct strains of NiV, Bangladesh (NiVB) and Malaysia (NiVM), and took 30 min from sample to result, suggesting these tests are well suited for rapid diagnosis under resource-limited conditions due to rapidity, simplicity, and low equipment requirements. These Nipah tests represent a first step toward development of near-patient NiV diagnostics that are appropriately sensitive for first-line screening, sufficiently robust for a range of peripheral settings, with potential to be safely performed outside of biohazard containment facilities.
Details
- Title
- Evaluation of three rapid low-resource molecular tests for Nipah virus
- Authors
- Nina Pollak (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationMalin Olsson (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringGlenn Marsh (Author) - Commonwealth Scientific and Industrial Research OrganisationJoanne Macdonald (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationDavid McMillan (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- Frontiers in Microbiology, Vol.13, pp.1-12
- Publisher
- Frontiers Research Foundation
- Date published
- 2023
- DOI
- 10.3389/fmicb.2022.1101914
- ISSN
- 1664-302X
- Copyright note
- © 2023 Pollak, Olsson, Marsh, Macdonald and McMillan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99704998402621
- Output Type
- Journal article
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