Journal article
Evaluation of the methods for enumerating coliform bacteria from water samples using precise reference standards
Letters in Applied Microbiology, Vol.42(4), pp.350-356
2006
Abstract
To use BioBallTM cultures as a precise reference standard to evaluate methods for enumeration of Escherichia coli and other coliform bacteria in water samples. Methods and Results: Eight methods were evaluated including membrane filtration, standard plate count (pour and spread plate methods), defined substrate technology methods (ColilertTM and ColisureTM), the most probable number method and the Petrifilm disposable plate method. Escherichia coli and Enterobacter aerogenes BioBallTM cultures containing 30 organisms each were used. All tests were performed using 10 replicates. The mean recovery of both bacteria varied with the different methods employed. Conclusions: The best and most consistent results were obtained with Petrifilm and the pour plate method. Other methods either yielded a low recovery or showed significantly high variability between replicates. Significance and Impact of the Study: The BioBallTM is a very suitable quality control tool for evaluating the efficiency of methods for bacterial enumeration in water samples.
Details
- Title
- Evaluation of the methods for enumerating coliform bacteria from water samples using precise reference standards
- Authors
- Tracey D Wohlsen (Author) - Queensland Health Scientific ServicesJ Bates (Author) - Queensland Health Scientific ServicesG Vesey (Author) - BTF Pty LtdWayne A Robinson (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationMohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
- Publication details
- Letters in Applied Microbiology, Vol.42(4), pp.350-356
- Publisher
- Blackwell Publishing Ltd.
- DOI
- 10.1111/j.1472-765X.2006.01854.x
- ISSN
- 0266-8254
- Organisation Unit
- Centre for Bioinnovation; School of Science, Technology and Engineering; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Faculty of Science, Health, Education and Engineering
- Language
- English
- Record Identifier
- 99449853902621
- Output Type
- Journal article
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