Evaluation of tetracyline, erythromycin, penicillin and streptomycin for decontaminating koala semen contaminated in vitro with chlamydiae

T J Bodetti; K Hengstberger; S Johnston; Peter Timms

doi:10.1136/vr.153.19.588

Back

Evaluation of tetracyline, erythromycin, penicillin and streptomycin for decontaminating koala semen contaminated in vitro with chlamydiae

Journal article

Peer reviewed

Evaluation of tetracyline, erythromycin, penicillin and streptomycin for decontaminating koala semen contaminated in vitro with chlamydiae

T J Bodetti, K Hengstberger, S Johnston and Peter Timms

Veterinary Record, Vol.153(19), pp.588-591

2003

DOI: https://doi.org/10.1136/vr.153.19.588

Files and links (1)

url

https://doi.org/10.1136/vr.153.19.588View

Published Version

Abstract

Semen from seven koalas was extended in a tris-citrate glucose diluent containing one of four antibiotics at different concentrations and then contaminated with a standard concentration of chlamydiae. These semen preparations were then tested for residual chlamydial viability by an in vitro cell culture assay, and any detrimental effect of the antibiotics on the motility and viability of the sperm was assessed. Penicillin at 25 iu/ml or more, erythromycin at 1000 ?g/ml or more and tetracycline at 2000 ?g/ml or more were highly effective at rendering the chlamydiae non-viable, but streptomycin showed no antichlamydial activity. There was a significant reduction of the motility of spermatozoa extended in diluents containing erythromycin (P<0.05), but spermatozoa incubated with tetracycline up to concentrations of 200 ?g/ml were not affected.

Details

Title: Evaluation of tetracyline, erythromycin, penicillin and streptomycin for decontaminating koala semen contaminated in vitro with chlamydiae
Authors: T J Bodetti (Author) - Queensland University of Technology
K Hengstberger (Author) - University of Queensland
S Johnston (Author) - University of Queensland
Peter Timms (Author) - Queensland University of Technology
Publication details: Veterinary Record, Vol.153(19), pp.588-591
Publisher: B M J Group
Date published: 2003
DOI: 10.1136/vr.153.19.588
ISSN: 0042-4900
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449982702621
Output Type: Journal article

Metrics

460 Record Views

4 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Veterinary Sciences

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites