Evaluation of multiple sewage-associated Bacteroides PCR markers for sewage pollution tracking

Warish Ahmed; A Goonetilleke; Daniel Powell; Ted Gardner

doi:10.1016/j.watres.2009.08.042

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Evaluation of multiple sewage-associated Bacteroides PCR markers for sewage pollution tracking

Journal article

Open access

Peer reviewed

Evaluation of multiple sewage-associated Bacteroides PCR markers for sewage pollution tracking

Warish Ahmed, A Goonetilleke, Daniel Powell and Ted Gardner

Water Research, Vol.43(19), pp.4872-4877

2009

DOI: https://doi.org/10.1016/j.watres.2009.08.042

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Abstract

feral pollution

water quality

microbial source tracking

bacteroides

sewage-associated markers

The host specificity of the five published sewage-associated Bacteroides markers (i.e., HF183, BacHum, HuBac, BacH and Human-Bac) was evaluated in Southeast Queensland, Australia by testing fecal DNA samples (n = 186) from 11 animal species including human fecal samples collected via influent to a sewage treatment plant (STP). All human fecal samples (n = 50) were positive for all five markers indicating 100% sensitivity of these markers. The overall specificity of the HF183 markers to differentiate between humans and animals was 99%. The specificities of the BacHum and BacH markers were > 94%, suggesting that these markers are suitable for the detection of sewage pollution in environmental waters in Australia. The HuBac (i.e., 63%) and Human-Bac (i.e., 79% specificity) markers performed poorly in distinguishing between the sources of human and animal fecal samples. It is recommended that the specificity of the sewage-associated markers must be rigorously tested prior to its application to identify the sources of fecal pollution in environmental waters.

Details

Title: Evaluation of multiple sewage-associated Bacteroides PCR markers for sewage pollution tracking
Authors: Warish Ahmed (Author) - Queensland Department of Natural Resources and Water
A Goonetilleke (Author) - Queensland University of Technology
Daniel Powell (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Ted Gardner (Author) - Queensland Department of Natural Resources and Water
Publication details: Water Research, Vol.43(19), pp.4872-4877
Publisher: IWA Publishing
Date published: 2009
DOI: 10.1016/j.watres.2009.08.042
ISSN: 0043-1354
Copyright note: Copyright © IWA Publishing 2009. The definitive peer-reviewed and edited version of this article is published in Water Research 43 (19) pp.4872-4877, 2009, http://dx.doi.org/10.1016/j.watres.2009.08.042 and is available at www.iwapublishing.com
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; Office of Research; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450168302621
Output Type: Journal article

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