Evaluation of fermentation conditions triggering increased antibacterial activity from a near-shore marine intertidal environment-associated Streptomyces species

Adrian L English; A Boufridi; R J Quinn; D Ipek Kurtboke

doi:10.1016/j.synbio.2016.09.005

Back

Evaluation of fermentation conditions triggering increased antibacterial activity from a near-shore marine intertidal environment-associated Streptomyces species

Journal article

Open access

Peer reviewed

Evaluation of fermentation conditions triggering increased antibacterial activity from a near-shore marine intertidal environment-associated Streptomyces species

Adrian L English, A Boufridi, R J Quinn and D Ipek Kurtboke

Synthetic and Systems Biotechnology, Vol.2(1), pp.28-38

2017

DOI: https://doi.org/10.1016/j.synbio.2016.09.005

Files and links (2)

pdf

PDF - Published Version (Open Access)2.84 MBDownload View

Published VersionPDF - Published Version (Open Access)CC BY-NC-ND V4.0, Open Access

url

https://doi.org/10.1016/j.synbio.2016.09.005View

Published Version

Abstract

marine streptomycetes

marine biodiscovery

fermentation media

antibiotics

marine natural products

pigments

A near-shore marine intertidal environment-associated Streptomyces isolate (USC-633) from the Sunshine Coast Region of Queensland, Australia, cultivated under a range of chemically defined and complex media to determine optimal parameters resulting in the secretion of diverse array of secondary metabolites with antimicrobial properties against various antibiotic resistant bacteria. Following extraction, fractioning and re-testing of active metabolites resulted in persistent antibacterial activity against Escherichia coli (Migula) (ATCC 13706) and subsequent Nuclear Magnetic Resonance (NMR) analysis of the active fractions confirmed the induction of metabolites different than the ones in fractions which did not display activity against the same bacterial species. Overall findings again confirmed the value of One Strain-Many Compounds (OSMAC) approach that tests a wide range of growth parameters to trigger bioactive compound secretion increasing the likelihood of finding novel therapeutic agents. The isolate was found to be adaptable to both marine and terrestrial conditions corresponding to its original near-shore marine intertidal environment. Wide variations in its morphology, sporulation and diffusible pigment production were observed when different growth media were used.

Details

Title: Evaluation of fermentation conditions triggering increased antibacterial activity from a near-shore marine intertidal environment-associated Streptomyces species
Authors: Adrian L English (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
A Boufridi (Author) - Griffith University
R J Quinn (Author) - Griffith University
D Ipek Kurtboke (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Synthetic and Systems Biotechnology, Vol.2(1), pp.28-38
Publisher: Ke Ai Publishing
Date published: 2017
DOI: 10.1016/j.synbio.2016.09.005
ISSN: 2405-805X
Copyright note: Copyright © 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Organisation Unit: School of Health - Biomedicine; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450318602621
Output Type: Journal article

Metrics

51 File views/ downloads

1411 Record Views

19 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites