Journal article
Evaluation of Bacteroides markers for the detection of human faecal pollution
Letters in Applied Microbiology, Vol.46(2), pp.237-242
2008
Abstract
Aims: This paper reports on the results of a study aimed at evaluating the specificity and sensitivity of human-specific HF183 and HF134 Bacteroides markers in various host groups and their utility to detect human faecal pollution in storm water samples collected from nonsewered catchments in Southeast Queensland, Australia. Methods and Results: The specificity and sensitivity of the HF183 and HF134 Bacteroides markers was evaluated by testing 207 faecal samples from 13 host groups, including 52 samples from human sources (via sewage and septic tanks). Polymerase chain reaction analysis of these samples revealed the presence/absence of HF183 and HF134 across these host groups, demonstrating their suitability for distinguishing between human and animal faecal pollution. The HF183 marker was found to be more reliable than that of HF134, which was also found in dogs. Conclusions: Based on our data, it appears that the HF183 marker is specific to sewage and is a reliable marker for detecting human faecal pollution, while the use of HF134 marker alone may not be sufficient enough to provide the evidence of human faecal pollution. Significance and Impact of the Study: This is the first study in Australia that rigorously evaluated the specificity and sensitivity of Bacteroides markers. Based on our findings, we suggest that the HF183 marker could reliably be used to detect the sources of human faecal pollution in Southeast Queensland region.
Details
- Title
- Evaluation of Bacteroides markers for the detection of human faecal pollution
- Authors
- Warish Ahmed (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationJ Stewart (Author) - Queensland Department of Natural Resources and WaterDaniel Powell (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationTed Gardner (Author) - Queensland Department of Natural Resources and Water
- Publication details
- Letters in Applied Microbiology, Vol.46(2), pp.237-242
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2008
- DOI
- 10.1111/j.1472-765X.2007.02287.x
- ISSN
- 0266-8254
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; Office of Research; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449149502621
- Output Type
- Journal article
Metrics
3 File views/ downloads
491 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites