Journal article
Evaluating the Prevent Effect of Human Papillomavirus 16E7 Peptide-Based Therapeutic Vaccine Utilizing an Orthotopic TC-1 Cervical Tumour Model by 3.0T Magnetic Resonance Imaging in Mice
Austin Journal of Radiology, Vol.7(1), 1106
2020
Abstract
The goal of this paper is to investigate whether a 3.0T magnetic resonance scanner with a small animal coil can monitor the orthotopic TC-1 cell Cervical Tumour (CT) growth in mice, and to evaluate the tumour growth prevent effect of Human Papillomavirus (HPV)16E7 peptide-based therapeutic vaccine. The TC-1 cells were implanted into cervical cavity of 52 female C57BL/6J mice (6 experiments), and followed by subcutaneously immunization of HPV16E7 peptide-based with interleukin 10 receptor antibody in 18 mice (3 groups) to observe the efficacy of the therapeutic vaccine. The same Magnetic Resonance Imaging (MRI) equipment and protocols were performed through the entire experiments. Images of each tumour-bearing mice were compared with blank mice to determine when the CTs can be determined by MRI. The Tumour Volume (TV) of each mice was monitored including 3 contrast enhanced tumors. The TV changes were evaluated by comparing the size on the same coronal MR images, and Signal Intensity (SI) measurement was adopted on 6 mice to predict the tumour growth trend. This study showed that the average CTs success rate was 90.38% (47/52), the tumour could be clearly detected on average 10 days. The maximum average SI values of tumour-bearing mice on day 4 was 2447, which was significantly higher than that of the blank mice, and increasing from day4 to day12. This study concluded that clinical 3.0T MRI scanner with small animal coil can be used to monitor the CTs, and to evaluate the CTs growth inhibitory effect of HPV16E7 peptide-based therapeutic vaccine, SI values may be used to predict cervical tumour growth and reliable than visual observation of early tumour images.
Details
- Title
- Evaluating the Prevent Effect of Human Papillomavirus 16E7 Peptide-Based Therapeutic Vaccine Utilizing an Orthotopic TC-1 Cervical Tumour Model by 3.0T Magnetic Resonance Imaging in Mice
- Authors
- X H He (Author) - First People's Hospital of Foshan, ChinaY H Chen (Author) - First People's Hospital of Foshan, ChinaL W Huang (Author) - First People's Hospital of Foshan, ChinaX H Zhou (Author) - First People's Hospital of Foshan, ChinaGuoying Ni (Author) - University of the Sunshine CoastTianfang Wang (Author) - University of the Sunshine CoastShelley F Walton (Author) - University of the Sunshine CoastXiao Song Liu (Author) - University of the Sunshine CoastS Chen (Author) - First People's Hospital of Foshan, ChinaM Y Gao (Author) - First People's Hospital of Foshan, China
- Publication details
- Austin Journal of Radiology, Vol.7(1), 1106; 8
- Publisher
- Austin Publishing Group
- Date published
- 2020
- ISSN
- 2473-0637; 2473-0637
- Copyright note
- Copyright © 2020. Reproduced with permission.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450958502621
- Output Type
- Journal article
- Research Statement
- false
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