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Published VersionCC BY V4.0, Open Access
Journal article
Evaluating conserved domains and motifs of decapod gonadotropinreleasing hormone G protein-coupled receptor superfamily
Frontiers in Endocrinology, Vol.15, pp.1-13
2024
Abstract
G protein-coupled receptors (GPCRs) are an ancient family of signal transducers that are both abundant and consequential in metazoan endocrinology. The evolutionary history and function of the GPCRs of the decapod superfamilies of gonadotropin-releasing hormone (GnRH) are yet to be fully elucidated.As part of which, the use of traditional phylogenetics and the recycling of a diminutive set of misannotated databases has proven insufficient. To address this, we have collated and revised eight existing and three novel GPCR repertoires for GnRH of decapod species. We developed a novel bioinformatic workflow that included clustering analysis to capture likely GnRH receptor-like proteins, followed by phylogenetic analysis of the seven transmembrane-spanning domains. A high degree of conservation of the sequences and topology of the domains and motifs allowed the identification of species-specific variation (up to ~70%, especially in the extracellular loops) that is thought to be influential to ligand-binding and function. Given the key functional role of the DRY motif across GPCRs, the classification of receptors based on the variation of this motif can be universally applied to resolve cryptic GPCR families, as was achieved in this work. Our results contribute to the resolution of the evolutionary history of invertebrate GnRH receptors and inform the design of bioassays in their deorphanization and functional annotation.
Details
- Title
- Evaluating conserved domains and motifs of decapod gonadotropinreleasing hormone G protein-coupled receptor superfamily
- Authors
- Sean Buckley (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringTuan Viet Nguyen (Author) - Agriculture VictoriaScott Cummins (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationAbigail Elizur (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationQuinn P Fitzgibbon (Author) - University of TasmaniaGreg Smith (Author) - University of TasmaniaDonald L Mykles (Corresponding Author) - Colorado State UniversityTomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- Frontiers in Endocrinology, Vol.15, pp.1-13
- Publisher
- Frontiers Research Foundation
- Date published
- 2024
- DOI
- 10.3389/fendo.2024.1348465
- ISSN
- 1664-2392
- Copyright note
- © 2024 Buckley, Viet Nguyen, Cummins, Elizur, Fitzgibbon, Smith, Mykles and Ventura. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99999995902621
- Output Type
- Journal article
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- Domestic collaboration
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- Web Of Science research areas
- Endocrinology & Metabolism
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