Establishment of an alphavirus-specific neutralization assay to distinguish infections with different members of the semliki forest complex

L Henss; C Yue; J Kandler; Helen M Faddy; G Simmons; M Panning; L L Lewis-Ximenez; S A Baylis; B S Schnierle

doi:10.3390/v11010082

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Establishment of an alphavirus-specific neutralization assay to distinguish infections with different members of the semliki forest complex

Journal article

Open access

Peer reviewed

Establishment of an alphavirus-specific neutralization assay to distinguish infections with different members of the semliki forest complex

L Henss, C Yue, J Kandler, Helen M Faddy, G Simmons, M Panning, L L Lewis-Ximenez, S A Baylis and B S Schnierle

Viruses, Vol.11(1), 82

2019

DOI: https://doi.org/10.3390/v11010082

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Abstract

Microbiology

alphavirus

neutralization assay

lentiviral vector

pseudotyping

Background: Alphaviruses are transmitted by arthropod vectors and can be found worldwide. Alphaviruses of the Semliki Forest complex such as chikungunya virus (CHIKV), Mayaro virus (MAYV) or Ross River virus (RRV) cause acute febrile illness and long-lasting arthralgia in humans, which cannot be clinically discriminated from a dengue virus or Zika virus infection. Alphaviruses utilize a diverse array of mosquito vectors for transmission and spread. For instance, adaptation of CHIKV to transmission by Aedes albopictus has increased its spread and resulted in large outbreaks in the Indian Ocean islands. For many alphaviruses commercial diagnostic tests are not available or show cross-reactivity among alphaviruses. Climate change and globalization will increase the spread of alphaviruses and monitoring of infections is necessary and requires virus-specific methods. Method: We established an alphavirus neutralization assay in a 384-well format by using pseudotyped lentiviral vectors. Results: MAYV-specific reactivity could be discriminated from CHIKV reactivity. Human plasma from blood donors infected with RRV could be clearly identified and did not cross-react with other alphaviruses. Conclusion: This safe and easy to use multiplex assay allows the discrimination of alphavirus-specific reactivity within a single assay and has potential for epidemiological surveillance. It might also be useful for the development of a pan-alphavirus vaccine.

Details

Title: Establishment of an alphavirus-specific neutralization assay to distinguish infections with different members of the semliki forest complex
Authors: L Henss (Author) - Paul-Ehrlich-Institut, Germany
C Yue (Author) - Paul-Ehrlich-Institut, Germany
J Kandler (Author) - Paul-Ehrlich-Institut, Germany
Helen M Faddy (Author) - Australian Red Cross Blood Service
G Simmons (Author)
M Panning (Author)
L L Lewis-Ximenez (Author)
S A Baylis (Author) - Paul-Ehrlich-Institut, Germany
B S Schnierle (Author) - Paul-Ehrlich-Institut, Germany
Publication details: Viruses, Vol.11(1), 82; 13
Publisher: MDPI AG
Date published: 2019
DOI: 10.3390/v11010082
ISSN: 1999-4915
Copyright note: Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450834302621
Output Type: Journal article

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