Journal article
Epidermal growth factor receptor in the prawn Macrobrachium rosenbergii: function and putative signaling cascade
Endocrinology, Vol.154(9), pp.3188-3196
2013
Abstract
Epidermal growth factor receptors (EGFRs) are highly conserved members of the tyrosine kinase receptor superfamily found in metazoans and plants. In arthropods, EGFRs are vital for the proper development of embryos and of adult limbs, gonads and eyes, as well as affecting body size. In searching for genes involved in the growth and development of our model organism, the decapod crustacean Macrobrachium rosenbergii, a comprehensive transcript library was established using next-generation sequencing. Using this library, the expression of several genes assigned to the signal transduction pathways mediated by EGFRs was observed, including a transcript encoding M. rosenbergii EGFR (Mr-EGFR), several potential ligands upstream to the receptor and most of the putative downstream signal transducer genes. The deduced protein encoded by Mr-EGFR, representing the first such receptor reported thus far in crustaceans, shows sequence similarity to other arthropod EGFRs. The M. rosenbergii gene is expressed in most tested tissues. The role of Mr-EGFR was revealed by temporarily silencing the transcript through weekly injections of double-stranded Mr-EGFR RNA. Such treatment resulted in a significant reduction in growth and a delay in the appearance of a male secondary sexual characteristic, namely the appendix masculina. An additional function of Mr-EGFR was revealed with respect to eye development. While the optic ganglion appeared to have retained its normal morphology, Mr-EGFR-silenced individuals developed abnormal eyes that presented irregular organization of the ommatidia, reflected by unorganized receptor cells occupying large areas of the dioptric portion and by a shortened crystalline tract layer.
Details
- Title
- Epidermal growth factor receptor in the prawn Macrobrachium rosenbergii: function and putative signaling cascade
- Authors
- O Sharabi (Author) - Ben-Gurion University of the Negev, IsraelTomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringR Manor (Author) - Ben-Gurion University of the Negev, IsraelE D Aflalo (Author) - Ben-Gurion University of the Negev, IsraelA Sagi (Author) - Ben-Gurion University of the Negev, Israel
- Publication details
- Endocrinology, Vol.154(9), pp.3188-3196
- Publisher
- Endocrine Society
- Date published
- 2013
- DOI
- 10.1210/en.2013-1259
- ISSN
- 0013-7227
- Copyright note
- Copyright © 2013 by The Endocrine Society. Reproduced with permission of the copyright holder.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448887002621
- Output Type
- Journal article
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