Encapsulation technology for short-term preservation and germplasm distribution of the African mahogany Khaya senegalensis

Cao Dinh Hung; Stephen J Trueman

doi:10.1007/s11240-011-9990-y

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Encapsulation technology for short-term preservation and germplasm distribution of the African mahogany Khaya senegalensis

Journal article

Open access

Peer reviewed

Encapsulation technology for short-term preservation and germplasm distribution of the African mahogany Khaya senegalensis

Cao Dinh Hung and Stephen J Trueman

Plant Cell, Tissue and Organ Culture, Vol.107(3), pp.397-405

2011

DOI: https://doi.org/10.1007/s11240-011-9990-y

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Abstract

adventitious roots

conversion

Meliaceae

plantlet

synthetic seeds

tissue culture

A protocol was developed for short-term preservation and distribution of the medicinal and timber plantation tree, Khaya senegalensis, using alginate-encapsulated shoot tips. The study assessed the effects of culture medium, storage temperature, auxin concentration and planting substrate on shoot regrowth or conversion into plantlets of four different clones. Optimal shoot growth was obtained, with high frequencies (92–100%) of shoot emergence, on Murashige and Skoog (MS) culture media containing 4.4 μM benzyladenine (BA). Encapsulated shoot tips survived longer at 25°C than at 4°C, with viability of 73–88% after 8 weeks. Conversion into plantlets was achieved on half-strength MS medium by pre-culture treatment of shoot tips with 49–490 μM indole-3-butyric acid (IBA) before encapsulation. Treatment with 245 μM IBA provided 52–98% conversion, and 90–95% of plantlets survived acclimatisation under nursery conditions. To eliminate the in vitro culture step after encapsulation, synthetic seeds were allowed to pre-convert before sowing directly onto a range of ex vitro non-sterile substrates. Highest frequencies of plantlet formation from pre-converted synthetic seeds (42–86%) were obtained by transferring synthetic seeds to organic compost, and these plantlets exhibited almost 100% survival in the nursery without mist irrigation. Pre-conversion is a novel and convenient method for producing synthetic seeds that are suitable for distribution to commercial nurseries.

Details

Title: Encapsulation technology for short-term preservation and germplasm distribution of the African mahogany Khaya senegalensis
Authors: Cao Dinh Hung (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Stephen J Trueman (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: Plant Cell, Tissue and Organ Culture, Vol.107(3), pp.397-405
Publisher: Springer Netherlands
Date published: 2011
DOI: 10.1007/s11240-011-9990-y
ISSN: 0167-6857
Copyright note: Copyright © 2011 Springer Netherlands. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final definitive publication is available at www.springerlink.com
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
Language: English
Record Identifier: 99449736402621
Output Type: Journal article

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