Encapsulation of sulfate-reducing bacteria in a silica host

K S Finnie; John R Bartlett; J L Woolfrey

doi:10.1039/A909350H

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Encapsulation of sulfate-reducing bacteria in a silica host

Journal article

Peer reviewed

Encapsulation of sulfate-reducing bacteria in a silica host

K S Finnie, John R Bartlett and J L Woolfrey

Journal of Materials Chemistry, Vol.10(5), pp.1099-1101

2000

DOI: https://doi.org/10.1039/A909350H

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Abstract

Engineering

Chemical Sciences

Anaerobic sulfate-reducing bacteria (SRB) have been encapsulated in a hydrous, alcohol-free, silica matrix produced by acidifying an aqueous mixture of colloidal silica and an SRB culture. The viability of the encapsulated species was investigated by monitoring the formation of the metabolic products, H2S and acetate. Freshly prepared gel samples containing ~5 × 105 cells cm-3 of gel reduced sulfate ions at a rate of ~11 μg h-1 cm-3 gel, when placed in a nutrient solution based on Postgate's Medium C. Less than 0.1% of the encapsulated species were leached into the nutrient solution over a 10 day period. Gels drained of nutrient solution and stored under nitrogen for 10 weeks at ambient temperature initially exhibited low sulfate reduction rates (~2 μg h-1 cm-3 gel) on re-immersion in nutrient, presumably due to a decrease in the encapsulated cell population. However, the initial sulfate reduction rate of ~11 μg h-1 cm-3 of gel was re-established after soaking the gel samples in nutrient solution for six days, indicating that the encapsulated bacterial population could be rapidly restored within the gel's pore network, even after several months of storage.

Details

Title: Encapsulation of sulfate-reducing bacteria in a silica host
Authors: K S Finnie (Author) - Australian Nuclear Science and Technology Organisation Materials Department
John R Bartlett (Author) - Australian Nuclear Science and Technology Organisation Materials Department
J L Woolfrey (Author) - Australian Nuclear Science and Technology Organisation Materials Department
Publication details: Journal of Materials Chemistry, Vol.10(5), pp.1099-1101
Publisher: Royal Society of Chemistry (R S C) Publications
Date published: 2000
DOI: 10.1039/A909350H
ISSN: 0959-9428
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99448731702621
Output Type: Journal article

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