Journal article
Embryo transfer cannot delineate between the maternal pregnancy environment and germ line effects in the transgenerational transmission of disease in rats
American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Vol.306(8), pp.R607-R618
2014
PMCID: PMC4043132
PMID: 24523338
Abstract
Embryo-transfer cannot delineate between maternal pregnancy environment and germ line effects in the transgenerational transmission of disease in rats. Am J Physiol Regul Integr Comp Physiol 306:R607-R618, 2014. First published February 12, 2014; doi:10.1152/ajpregu. 00523.2013.-Adverse conditions in utero can have transgenerational effects, in the absence of a subsequent insult. We aimed to investigate the contribution of the maternal pregnancy environment vs. germ line effects in mediating alterations to cardiorenal and metabolic physiology in offspring from mothers born small. Uteroplacental insufficiency was induced by bilateral uterine artery and vein ligation (Restricted group) or sham surgery (Control group) in Wistar-Kyoto rats. Restricted and control female offspring (F1) were mated with either breeder males (embryo donor) or vasectomized males (embryo recipient). Embryo transfer was performed at embryonic day (E) 1, whereby second-generation (F2) embryos gestated (donor-in-recipient) in either a control (Cont-in-Cont, Rest-in-Cont) or restricted (Cont-in-Rest, Rest-in-Rest) mother. In male and female offspring, glomerular number and size were measured at postnatal day (PN) 35, and systolic blood pressure, glucose control, insulin sensitivity, and pancreatic beta-cell mass were measured in separate sibling cohorts at 6 mo. Rest-in-Rest offspring were hypothesized to have similar characteristics (reduced growth, altered metabolic control, and hypertension) to non-embryo-transferred Rest, such that embryo transfer would not be a confounding experimental influence. However, embryo-transferred Rest-in-Rest offspring underwent accelerated growth during the peripubertal phase, followed by slowed growth between 2 and 3 mo of age compared with non-embryo-transferred Rest groups. Furthermore, renal function and insulin response to a glucose load were different to respective non-embryo- transferred groups. Our data demonstrate the long-term effects of in vitro embryo manipulation, which confounded the utility of this approach in delineating between the maternal pregnancy environment and germ line effects that drive transgenerational outcomes.
Details
- Title
- Embryo transfer cannot delineate between the maternal pregnancy environment and germ line effects in the transgenerational transmission of disease in rats
- Authors
- Melanie Tran (Author) - University of MelbourneLinda A. Gallo (Author) - University of MelbourneAlanna N. Hanvey (Author) - University of MelbourneAndrew J. Jefferies (Author) - University of MelbourneKerryn T. Westcott (Author) - University of MelbourneLuise A. Cullen-McEwen (Author) - Monash UniversityDavid K. Gardner (Author) - Univ Melbourne, Dept Zool, Parkville, Vic 3010, AustraliaKaren M. Moritz (Author) - University of QueenslandMary E. Wlodek (Corresponding Author) - University of Melbourne
- Publication details
- American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Vol.306(8), pp.R607-R618
- Publisher
- American Physiological Society
- DOI
- 10.1152/ajpregu.00523.2013
- ISSN
- 1522-1490
- PMID
- 24523338; PMC4043132
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy; School of Health - Biomedicine
- Language
- English
- Record Identifier
- 99679194302621
- Output Type
- Journal article
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