Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Solenostemon scutellarioides

R Sahu; Moumita Gangopadhyay; S Dewanjee

doi:10.1007/s11738-012-1188-3

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Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Solenostemon scutellarioides

Journal article

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Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Solenostemon scutellarioides

R Sahu, Moumita Gangopadhyay and S Dewanjee

Acta Physiologiae Plantarum, Vol.35(5), pp.1473-1481

2013

DOI: https://doi.org/10.1007/s11738-012-1188-3

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Abstract

rosmarinic acid

solenostemon scutellarioides

elicitors

enzyme activity

biosynthetic pathway

This study aimed to improve rosmarinic acid (RA) production in the whole plant culture of Solenostemon scutellarioides through elicitation. Amongst selected elicitors methyl jasmonate (MJ), salicylic acid (SA), and yeast extract (YE) caused significant elevation in RA accumulation. Elicitation with MJ (50 μM) and SA (50 μM) caused almost 1.7 and 1.4-fold increase in RA accumulation, respectively, within day 1. While YE (100 μg ml−1) elicitation showed highest RA content (~1.5-fold) in day 3. Preceding the elicitor-induced RA accumulation, there was a notable alteration in the specific activities of RA biosynthetic enzymes viz. phenylalanine ammonia lyase, tyrosine aminotransferase, hydroxyl-phenylpyruvate reductase and rosmarinic acid synthase up on MJ (50 μM), SA (50 μM) and YE (100 mg ml−1) elicitation. Based on differential responses of aforementioned enzymes, RA synthesis was further scaled up through combination of elicitors in pre-optimized doses. In synergy study, at a time exposure with MJ + SA + YE and MJ + SA followed by YE after 24 h has been found to produce significant elevation of RA (2.0 and 1.9-fold, respectively) within 24 h while later maintained a steady state increased level (~1.7 ± 0.2-fold) over control up to day 7.

Details

Title: Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Solenostemon scutellarioides
Authors: R Sahu (Author) - Jadavpur University, India
Moumita Gangopadhyay (Author) - Jadavpur University, India
S Dewanjee (Author) - Jadavpur University, India
Publication details: Acta Physiologiae Plantarum, Vol.35(5), pp.1473-1481
Publisher: Springer Verlag
Date published: 2013
DOI: 10.1007/s11738-012-1188-3
ISSN: 0137-5881
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450226302621
Output Type: Journal article

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