Electrospun Pectin-Polyhydroxybutyrate Nanofibers for Retinal Tissue Engineering

Siew Yin Chan; Benjamin Qi Yu Chan; Zengping Liu; Bhav Harshad Parikh; Kangyi Zhang; Qianyu Lin; Xinyi Su; Dan Kai; Wee Sim Choo; David James Young; Xian Jun Loh

doi:10.1021/acsomega.7b01604

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Electrospun Pectin-Polyhydroxybutyrate Nanofibers for Retinal Tissue Engineering

Journal article

Open access

Peer reviewed

Electrospun Pectin-Polyhydroxybutyrate Nanofibers for Retinal Tissue Engineering

Siew Yin Chan, Benjamin Qi Yu Chan, Zengping Liu, Bhav Harshad Parikh, Kangyi Zhang, Qianyu Lin, Xinyi Su, Dan Kai, Wee Sim Choo, David James Young, …

ACS Omega, Vol.2(12), pp.8959-8968

2017

DOI: https://doi.org/10.1021/acsomega.7b01604

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Abstract

Natural polysaccharide pectin has for the first time been grafted with polyhydroxybutyrate (PHB) via ring-opening polymerization of β-butyrolactone. This copolymer, pectin-polyhydroxybutyrate (pec-PHB), was blended with PHB in various proportions and electrospun to produce nanofibers that exhibited uniform and bead-free nanostructures, suggesting the miscibility of PHB and pec-PHB. These nanofiber blends exhibited reduced fiber diameters from 499 to 336-426 nm and water contact angles from 123.8 to 88.2° on incorporation of pec-PHB. They also displayed 39-335% enhancement of elongation at break relative to pristine PHB nanofibers. pec-PHB nanofibers were found to be noncytotoxic and biocompatible. Human retinal pigmented epithelium (ARPE-19) cells were seeded onto pristine PHB and pec-PHB nanofibers as scaffold and showed good proliferation. Higher proportions of pec-PHB (pec-PHB10 and pec-PHB20) yielded higher densities of cells with similar characteristics to normal RPE cells. We propose, therefore, that nanofibers of pec-PHB have significant potential as retinal tissue engineering scaffold materials.

Details

Title: Electrospun Pectin-Polyhydroxybutyrate Nanofibers for Retinal Tissue Engineering
Authors: Siew Yin Chan (Author) - Monash University Malaysia, Malaysia
Benjamin Qi Yu Chan (Author) - Institute of Materials Research and Engineering (IMRE), Singapore
Zengping Liu (Author) - National University of Singapore, Singapore
Bhav Harshad Parikh (Author) - National University of Singapore, Singapore
Kangyi Zhang (Author) - Institute of Materials Research and Engineering (IMRE), Singapore
Qianyu Lin (Author) - Institute of Materials Research and Engineering (IMRE), Singapore
Xinyi Su (Author) - National University of Singapore, Singapore
Dan Kai (Author) - Institute of Materials Research and Engineering (IMRE), Singapore
Wee Sim Choo (Author) - Monash University Malaysia, Malaysia
David James Young (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Xian Jun Loh (Author) - Institute of Materials Research and Engineering (IMRE), Singapore
Publication details: ACS Omega, Vol.2(12), pp.8959-8968
Publisher: American Chemical Society
Date published: 2017
DOI: 10.1021/acsomega.7b01604
ISSN: 2470-1343
Copyright note: Copyright © 2017 The Authors. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450889902621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Chemistry, Multidisciplinary

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