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Electroimmobilization of nitrate reductase and nicotinamide adenine dinucleotide into polypyrrole films for potentiometric detection of nitrate
Journal article   Peer reviewed

Electroimmobilization of nitrate reductase and nicotinamide adenine dinucleotide into polypyrrole films for potentiometric detection of nitrate

Manzar Sohail and S B Adeloju
Sensors and Actuators B: Chemical, Vol.133(1), pp.333-339
2008
DOI: https://doi.org/10.1016/j.snb.2008.02.032
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https://doi.org/10.1016/j.snb.2008.02.032View
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Abstract

nitrate biosensor nitrate reductase NADH potentiometry polypyrrole
Galvanostatic immobilization of nitrate reductase (NaR) with and without β-NADH, a biologically active form of nicotinic acid employed as a co-factor, into conducting polypyrrole (PPy) films has been successfully used to fabricate a novel potentiometric nitrate biosensor. The co-incorporation of NADH and NaR into PPy films resulted in substantial enhancement of the nitrate response compared to when present in solution. The formation of optimum PPy-NaR-NADH film for nitrate detection was accomplished with an applied current density of 0.5 mA cm-2, polymerization time of 4 min, 0.1 M pyrrole, 1 U/mL NaR, 0.3 M KCl and 0.3 mM NADH. Optimum potentiometric response for nitrate was obtained in 0.1 M phosphate buffer (pH 7.30). A minimum detectable concentration of 15 μM and a linear concentration range of 100-5000 μM were achieved with the nitrate biosensor, with a relative standard deviation of 1.9% (n = 6).

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Chemistry, Analytical
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