Journal article
Efficacy of Selected Live Biotherapeutic Candidates to Inhibit the Interaction of an Adhesive-Invasive Escherichia coli Strain with Caco-2, HT29-MTX Cells and Their Co-Culture
Biomedicines, Vol.10(9), pp.1-13
2022
PMCID: PMC9496071
PMID: 36140346
Abstract
Adherent-invasive Escherichia coli (AIEC) has been implicated as a microbiological factor in the pathogenesis of inflammatory bowel disease (IBD). We evaluated the ability of six live biotherapeutic products (LBPs) to inhibit the interaction of an AIEC strain to three cell lines representing human gut epithelium. Co-inoculation of LBPs with AIEC showed a reduction in adhesion (up to 73%) and invasion of AIEC (up to 89%). Pre-inoculation of LBPs in HT-29-MTX and Caco-2 cells before challenging with AIEC further reduced the adhesion and invasion of the AIEC, with three LBPs showing significantly (p < 0.0001) higher efficiency in reducing the adhesion of AIEC. In co-inoculation experiments, the highest reduction in adhesion (73%) of AIEC was observed in HT-29-MTX cells, whereas the highest reduction in invasion (89%) was seen in HT-29-MTX and the co-culture of cells. Pre-inoculation of LBPs further reduced the invasion of AIEC with highest reduction (97%) observed in co-culture of cells. Our results indicated that whilst there were differences in the efficacy of LBPs, they all reduced interaction of AIEC with cell lines representing gut epithelium. Their efficiency was higher when they were pre-inoculated onto the cells, suggesting their potential as candidates for alleviating pathogenesis of AIEC in patients with IBD.
Details
- Title
- Efficacy of Selected Live Biotherapeutic Candidates to Inhibit the Interaction of an Adhesive-Invasive Escherichia coli Strain with Caco-2, HT29-MTX Cells and Their Co-Culture
- Authors
- Bronwyn Smit (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationChiemeka C Chinaka (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationAlbert A Scott (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationKirsten Gaiduschek (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationEva Hatje (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationAnna Kuballa (Author) - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - LegacySamantha Coulson (Author) - Servatus BiopharmaceuticalsWayne Finlayson (Author) - Servatus BiopharmaceuticalsMohammad Katouli (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- Biomedicines, Vol.10(9), pp.1-13
- Publisher
- MDPI AG
- Date published
- 2022
- DOI
- 10.3390/biomedicines10092245
- ISSN
- 2227-9059
- PMID
- 36140346; PMC9496071
- Copyright note
- © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99676396302621
- Output Type
- Journal article
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