Efficacy of Selected Live Biotherapeutic Candidates to Inhibit the Interaction of an Adhesive-Invasive Escherichia coli Strain with Caco-2, HT29-MTX Cells and Their Co-Culture

Bronwyn Smit; Chiemeka C Chinaka; Albert A Scott; Kirsten Gaiduschek; Eva Hatje; Anna Kuballa; Samantha Coulson; Wayne Finlayson; Mohammad Katouli

doi:10.3390/biomedicines10092245

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Efficacy of Selected Live Biotherapeutic Candidates to Inhibit the Interaction of an Adhesive-Invasive Escherichia coli Strain with Caco-2, HT29-MTX Cells and Their Co-Culture

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Efficacy of Selected Live Biotherapeutic Candidates to Inhibit the Interaction of an Adhesive-Invasive Escherichia coli Strain with Caco-2, HT29-MTX Cells and Their Co-Culture

Bronwyn Smit, Chiemeka C Chinaka, Albert A Scott, Kirsten Gaiduschek, Eva Hatje, Anna Kuballa, Samantha Coulson, Wayne Finlayson and Mohammad Katouli

Biomedicines, Vol.10(9), pp.1-13

2022

DOI: https://doi.org/10.3390/biomedicines10092245

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Abstract

AEIC

live biotherapeutic products

Caco-2:HT-29-MTX

adhesion

invasion

Adherent-invasive Escherichia coli (AIEC) has been implicated as a microbiological factor in the pathogenesis of inflammatory bowel disease (IBD). We evaluated the ability of six live biotherapeutic products (LBPs) to inhibit the interaction of an AIEC strain to three cell lines representing human gut epithelium. Co-inoculation of LBPs with AIEC showed a reduction in adhesion (up to 73%) and invasion of AIEC (up to 89%). Pre-inoculation of LBPs in HT-29-MTX and Caco-2 cells before challenging with AIEC further reduced the adhesion and invasion of the AIEC, with three LBPs showing significantly (p < 0.0001) higher efficiency in reducing the adhesion of AIEC. In co-inoculation experiments, the highest reduction in adhesion (73%) of AIEC was observed in HT-29-MTX cells, whereas the highest reduction in invasion (89%) was seen in HT-29-MTX and the co-culture of cells. Pre-inoculation of LBPs further reduced the invasion of AIEC with highest reduction (97%) observed in co-culture of cells. Our results indicated that whilst there were differences in the efficacy of LBPs, they all reduced interaction of AIEC with cell lines representing gut epithelium. Their efficiency was higher when they were pre-inoculated onto the cells, suggesting their potential as candidates for alleviating pathogenesis of AIEC in patients with IBD.

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Title: Efficacy of Selected Live Biotherapeutic Candidates to Inhibit the Interaction of an Adhesive-Invasive Escherichia coli Strain with Caco-2, HT29-MTX Cells and Their Co-Culture
Authors: Bronwyn Smit (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Chiemeka C Chinaka (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Albert A Scott (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Kirsten Gaiduschek (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Eva Hatje (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Anna Kuballa (Author) - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - Legacy
Samantha Coulson (Author) - Servatus Biopharmaceuticals
Wayne Finlayson (Author) - Servatus Biopharmaceuticals
Mohammad Katouli (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Publication details: Biomedicines, Vol.10(9), pp.1-13
Publisher: MDPI AG
Date published: 2022
DOI: 10.3390/biomedicines10092245
ISSN: 2227-9059
Copyright note: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Organisation Unit: School of Health - Biomedicine; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99676396302621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Medicine, Research & Experimental; Pharmacology & Pharmacy

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