Journal article
Effects of neuropeptides and sex steroids on the pituitary-gonadal axis of pre-pubertal F1 wreckfish (hāpuku) Polyprion oxygeneios in vivo: evidence of inhibitory effects of androgens
General and Comparative Endocrinology, Vol.257, pp.113-121
2018
Abstract
The ability to advance puberty in broodstock that have a long generation interval and mature at large size is a highly valuable tool in contemporary aquaculture enterprise. Juvenile male and female wreckfish 'hāpuku' (Polyprion oxygeneios), a candidate for commercialization in aquaculture, were subjected to treatment for 8 weeks with two implants, one containing steroid (blank; estradiol-17β, E2; 11-ketotestosterone, KT; 17 α -methyltestosterone, MT), the other peptide (blank; gonadotropin-releasing hormone analog, GnRHa; kisspeptin, Kiss2-12). The expression of target genes (glycoprotein homone α -subunit, gpa; follicle stimulating-hormone β -subunit, fshb; luteinizing hormone β -subunit, lhb; GnRH receptor, gnrhr) in the pituitary was assayed by quantitative PCR. KT and MT decreased mRNA levels of all target genes in both male and female hāpuku, suggestive of a strong inhibitory tone by these steroid hormones. E2, GnRHa and Kiss2-12 were largely ineffective, regardless of whether they were administered alone or in combination with steroid implants. Clear differences in release and/or clearance rates between E2 and KT from implants were evident, in part explaining our observations. Advancement of puberty was not achieved, and we pose that different hormone doses and/or administration during more advanced stages of gonadogenesis need to be considered to move this field forward.
Details
- Title
- Effects of neuropeptides and sex steroids on the pituitary-gonadal axis of pre-pubertal F1 wreckfish (hāpuku) Polyprion oxygeneios in vivo: evidence of inhibitory effects of androgens
- Authors
- Matthew J Wylie (Author) - University of Otago, New ZealandAlvin N Setiawan (Author) - Northland Marine Research Centre, New ZealandGlen W Irvine (Author) - Northland Marine Research Centre, New ZealandJane E Symonds (Author) - Northland Marine Research Centre, New ZealandAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringP Mark Lokman (Author) - University of Otago, New Zealand
- Publication details
- General and Comparative Endocrinology, Vol.257, pp.113-121
- Publisher
- Academic Press
- Date published
- 2018
- DOI
- 10.1016/j.ygcen.2017.08.018
- ISSN
- 0016-6480
- Copyright note
- Copyright © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451034802621
- Output Type
- Journal article
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- Endocrinology & Metabolism
- Zoology
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