Effects of inoculating dose on the kinetics of Chlamydia muridarum genital infection in female mice

A J Carey; K A Cunningham; L M Hafner; Peter Timms; K W Beagley

doi:10.1038/icb.2009.3

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Effects of inoculating dose on the kinetics of Chlamydia muridarum genital infection in female mice

Journal article

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Effects of inoculating dose on the kinetics of Chlamydia muridarum genital infection in female mice

A J Carey, K A Cunningham, L M Hafner, Peter Timms and K W Beagley

Immunology and Cell Biology, Vol.87(4), pp.337-343

2009

DOI: https://doi.org/10.1038/icb.2009.3

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Abstract

Chlamydia infection

female reproductive tract

inflammation

Chlamydia trachomatis infections have been implicated in problems such as pelvic inflammatory disease and infertility in females. Although there are some studies examining the kinetics of ascending infection, there is limited information on the kinetics of pathology development and cellular infiltrate into the reproductive tissues in relation to the effects of inoculating dose, and a better understanding of these is needed. The murine model of female genital tract Chlamydia muridarum infection is frequently used as a model of human C. trachomatis reproductive tract infection. To investigate the kinetics of ascending genital infection and associated pathology development, female BALB/c mice were intravaginally infected with C. muridarum at doses ranging from 5 × 102 to 2.6 × 106 inclusion forming units. We found that the inoculating dose affects the course of infection and the ascension of bacteria, with the highest dose ascending rapidly to the oviducts. By comparison, the lowest dose resulted in the greatest bacterial load in the lower reproductive tract. Interestingly, we found that the dose did not significantly affect inflammatory cell infiltrate in the various regions. Overall, this data show the effects of infectious dose on the kinetics of ascending chlamydial infection and associated inflammatory infiltration in BALB/c mice.

Details

Title: Effects of inoculating dose on the kinetics of Chlamydia muridarum genital infection in female mice
Authors: A J Carey (Author) - Queensland University of Technology
K A Cunningham (Author) - Queensland University of Technology
L M Hafner (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
K W Beagley (Author) - Queensland University of Technology
Publication details: Immunology and Cell Biology, Vol.87(4), pp.337-343
Publisher: Nature Publishing Group
Date published: 2009
DOI: 10.1038/icb.2009.3
ISSN: 0818-9641
Copyright note: Copyright © 2009 Nature Publishing Group. The accepted version is reproduced here in accordance with the publishers copyright policy. The definitive version is available from http://www.nature.com/icb/
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99448856202621
Output Type: Journal article

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