Effects of frozen storage temperature on the elasticity of tendons from a small murine model

K L Goh; Y Chen; S M Chou; A Listrat; D Bechet; Tim J Wess

doi:10.1017/S1751731110000698

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Effects of frozen storage temperature on the elasticity of tendons from a small murine model

Journal article

Peer reviewed

Effects of frozen storage temperature on the elasticity of tendons from a small murine model

K L Goh, Y Chen, S M Chou, A Listrat, D Bechet and Tim J Wess

Animal, Vol.4(9), pp.1613-1617

2010

DOI: https://doi.org/10.1017/S1751731110000698

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Abstract

collagen

frozen storage temperature

stiffness

strain energy

strength

Murinae

The basic mechanism of reinforcement in tendons addresses the transfer of stress, generated by the deforming proteoglycan (PG)-rich matrix, to the collagen fibrils. Regulating this mechanism involves the interactions of PGs on the fibril with those in the surrounding matrix and between PGs on adjacent fibrils. This understanding is key to establishing new insights on the biomechanics of tendon in various research domains. However, the experimental designs in many studies often involved long sample preparation time. To minimise biological degradation the tendons are usually stored by freezing. Here, we have investigated the effects of commonly used frozen storage temperatures on the mechanical properties of tendons from the tail of a murine model (C57BL6 mouse). Fresh (unfrozen) and thawed samples, frozen at temperatures of 20C and 80C, respectively, were stretched to rupture. Freezing at 20C revealed no effect on the maximum stress (), stiffness (E), the corresponding strain () at and strain energy densities up to (u) and from until complete rupture (up). On the other hand, freezing at 80C led to higher , E and u; and up were unaffected. The results implicate changes in the long-range order of radially packed collagen molecules in fibrils, resulting in fibril rupture at higher stresses, and changes to the composition of extrafibrillar matrix, resulting in an increase in the interaction energy between fibrils via collagen-bound PGs. Copyright © The Animal Consortium 2010.

Details

Title: Effects of frozen storage temperature on the elasticity of tendons from a small murine model
Authors: K L Goh (Author) - Monash University
Y Chen (Author) - Nanyang Technological University, Singapore
S M Chou (Author) - Nanyang Technological University, Singapore
A Listrat (Author) - Institut National de la Recherche Agronomique, France
D Bechet (Author) - Institut National de la Recherche Agronomique, France
Tim J Wess (Author) - Cardiff University, United Kingdom
Publication details: Animal, Vol.4(9), pp.1613-1617
Publisher: Cambridge University Press
Date published: 2010
DOI: 10.1017/S1751731110000698
ISSN: 1751-7311
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450903602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Agriculture, Dairy & Animal Science; Veterinary Sciences

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