Journal article
Effects of dietary selenium on post-ischemic expression of antioxidant mRNA
Molecular and Cellular Biochemistry, Vol.270(1-2), pp.131-138
2005
PMID: 15792362
Abstract
Cardiac ischemia reperfusion leads to oxidative stress and poor physiological recovery. Selenium deficiency down-regulates thioredoxin reductase (Txnrd) and glutathione peroxidase (Gpx) activity, impairing recovery from ischemia-reperfusion. Furthermore, selenium supplementation has been shown to be cardioprotective and lessens oxidative stress in reperfused rat hearts. In this study we have investigated the role of selenium in the mRNA expression of these, and related antioxidant proteins, post ischemia-reperfusion. Male rats were fed varying doses of selenium for five weeks. Hearts were isolated and perfused using the Langendorff method with 22.5 min of global ischemia and 45 min reperfusion. RNA was extracted for quantitative real-time PCR analysis of glutathione peroxidase (Gpx)-1 and 4, glutathione reductase (Gsr), thioredoxin peroxidase-2 (Prdx2), thioredoxin (Txn) and thioredoxin reductase (Txnrd)-1 and 2 gene expression. Selenium deficiency produced significant reductions in Gpx-1, Gpx-4, Prdx2, Txnrd-1 and Txnrd-2 expression. Conversely, selenium supplementation of 1000 μg/kg significantly up-regulated Gpx-1, Gpx-4, Txn, Txnrd-1 and Txnrd-2 transcription. Our results show selenium modulates the cardiac mRNA expression of thioredoxin and glutathione related enzymes post ischemia-reperfusion, and impacts on tolerance to ischemia-reperfusion. (Mol Cell Biochem 270: 131-138, 2005)[PUBLICATION ABSTRACT]
Details
- Title
- Effects of dietary selenium on post-ischemic expression of antioxidant mRNA
- Authors
- Kylie Venardos (Author) - Griffith UniversityKevin Ashton (Author) - Griffith UniversityJohn Headrick (Author) - Griffith UniversityAnthony Perkins (Author) - Griffith University
- Publication details
- Molecular and Cellular Biochemistry, Vol.270(1-2), pp.131-138
- Publisher
- Springer New York LLC
- DOI
- 10.1007/s11010-005-5279-y
- ISSN
- 1573-4919
- PMID
- 15792362
- Organisation Unit
- School of Health and Behavioural Sciences - Legacy; School of Health
- Language
- English
- Record Identifier
- 99685193602621
- Output Type
- Journal article
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