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Effects of Pseudoloma neurophilia infection on the brain transcriptome in zebrafish (Danio rerio)1.29 MB
Published VersionCC BY V4.0, Open Access
Journal article
Effects of Pseudoloma neurophilia infection on the brain transcriptome in zebrafish (Danio rerio)
Journal of Fish Diseases, Vol.43(8), pp.863-875
2020
Abstract
Laboratory zebrafish are commonly infected with the intracellular, brain‐infecting microsporidian parasite Pseudoloma neurophilia. Chronic P. neurophilia infections induce inflammation in meninges, brain and spinal cord, and have been suggested to affect neural functions since parasite clusters reside inside neurons. However, underlying neural and immunological mechanisms associated with infection have not been explored. Utilizing RNA‐sequencing analysis, we found that P. neurophilia infection upregulated 175 and downregulated 45 genes in the zebrafish brain, compared to uninfected controls. Four biological pathways were enriched by the parasite, all of which were associated with immune function. In addition, 14 gene ontology (GO) terms were enriched, eight of which were associated with immune responses and five with circadian rhythm. Surprisingly, no differentially expressed genes or enriched pathways were specific for nervous system function. Upregulated immune‐related genes indicate that the host generally show a pro‐inflammatory immune response to infection. On the other hand, we found a general downregulation of immune response genes associated with anti‐pathogen functions, suggesting an immune evasion strategy by the parasite. The results reported here provide important information on host–parasite interaction and highlight possible pathways for complex effects of parasite infections on zebrafish phenotypes.
Details
- Title
- Effects of Pseudoloma neurophilia infection on the brain transcriptome in zebrafish (Danio rerio)
- Authors
- Helene L E Midttun (Corresponding Author) - Norwegian University of Life SciencesMarco A Vindas (Author) - Norwegian University of Life SciencesPaul Whatmore (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyØyvind Øverli (Author) - Norwegian University of Life SciencesIda B Johansen (Author) - Norwegian University of Life Sciences
- Publication details
- Journal of Fish Diseases, Vol.43(8), pp.863-875; 13
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2020
- DOI
- 10.1111/jfd.13198
- ISSN
- 1365-2761; 0140-7775
- Copyright note
- Copyright © 2020 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Grant note
- Norges Forskningsråd (250048)
- Organisation Unit
- University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
- Language
- English
- Record Identifier
- 99465406102621
- Output Type
- Journal article
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- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Fisheries
- Marine & Freshwater Biology
- Veterinary Sciences
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