Effects of Multiple Near‐Infrared LEDs (700, 850, and 980 nm) CW ‐ PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts

Simone Sleep; Deanne H. Hryciw; Laurence J. Walsh; Eliza Ranjit; Nifty Tomy; Praveen R. Arany; Roy George

doi:10.1002/jbio.70015

Back

Effects of Multiple Near‐Infrared LEDs (700, 850, and 980 nm) CW ‐ PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts

Journal article

Open access

Peer reviewed

Effects of Multiple Near‐Infrared LEDs (700, 850, and 980 nm) CW ‐ PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts

Simone Sleep, Deanne H. Hryciw, Laurence J. Walsh, Eliza Ranjit, Nifty Tomy, Praveen R. Arany and Roy George

Journal of Biophotonics, Vol.18(8), pp.1-10

2025

DOI: https://doi.org/10.1002/jbio.70015

PMID: 40177989

Files and links (1)

pdf

Journal of Biophotonics - 2025 - Sleep - Effects of Multiple Near‐Infrared LEDs 700 850 and 980 nm CW‐PBM on889.92 kBDownload View

Published VersionCC BY V4.0, Open Access

Abstract

gene regulation

LED

multi-wavelength

osteogenesis

photobiomodulation

spectral width

Background and Aim This study evaluated mitochondrial and osteogenic activity in MG-63 pre-osteoblastic cells after photobiomodulation (PBM) using multiple near-infrared LED sources (Nuralyte) emitting wavelengths from 700 to 1100 nm. Materials and Methods MG-63 cells were irradiated daily for 3, 5, or 7 days with energy densities of 5.3 J/cm2 (30 s, optimal dose) and 10.6 J/cm2 (60 s, high dose). Mitochondrial function was assessed using the XF Seahorse analyzer, and gene expression of osteogenic markers was analyzed. Results Maximal mitochondrial oxygen consumption rate (OCR) significantly decreased at the optimal dose but increased at the high dose (p < 0.001) in 5-day irradiated cultures. Upregulation of osteogenic markers (OCN, OPN, BMP-2, COL-1, RUNX2) occurred after 3–5 consecutive days of irradiation, with greater activation at the optimal dose. Conclusion MG-63 cells respond to PBM using MNI-LEDs (700, 850, 980 nm) by modulating mitochondrial respiration and boosting bone-related gene expression in a dose- and time-dependent manner.

Details

Title: Effects of Multiple Near‐Infrared LEDs (700, 850, and 980 nm) CW ‐ PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts
Authors: Simone Sleep - Griffith University
Deanne H. Hryciw - Griffith University
Laurence J. Walsh - The University of Queensland
Eliza Ranjit - Griffith University
Nifty Tomy - National Centre for Disease Informatics and Research
Praveen R. Arany - National Institute of Dental and Craniofacial Research
Roy George (Corresponding Author) - Griffith University
Publication details: Journal of Biophotonics, Vol.18(8), pp.1-10
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
Date published: 2025
DOI: 10.1002/jbio.70015
ISSN: 1864-0648; 1864-063X
PMID: 40177989
Copyright note: © 2025 The Author(s). Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Data Availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Organisation Unit: School of Health - Biomedicine
Language: English
Record Identifier: 991187228602621
Output Type: Journal article

Metrics

1 Record Views

See more details