Journal article
Effects of 4-nonylphenol and 17α-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata: Vitellogenin induction and gonadal development
Aquatic Toxicology, Vol.88(1), pp.39-47
2008
Abstract
Adult Saccostrea glomerata were exposed to environmentally relevant concentrations of 4-nonylphenol (1 μg/L and 100 μg/L) and 17α-ethynylestradiol (5 ng/L and 50 ng/L) in seawater over 8 weeks. Exposures were performed to assess effects on vitellogenin induction and gonadal development during reproductive conditioning. Chronic direct estrogenicity within gonadal tissue was assessed via an estrogen receptor-mediated, chemical-activated luciferase reporter gene-expression assay (ER-CALUX®). Estradiol equivalents (EEQ) were greatest in the 100 μg/L 4-nonylphenol exposure (28.7±2.3 ng/g tissue EEQ) while 17α-ethynylestradiol at concentrations of 50 ng/L were 2.2±1.5 ng/g tissue EEQ. Results suggest 4-nonylphenol may be accumulated in tissue and is partly resistant to biotransformation; maintaining its potential for chronic estrogenic action, while 17α-ethynylestradiol, although exhibiting greater estrogenic potency on biological endpoints possibly exerts its estrogenic action before being rapidly metabolised and/or excreted. A novel methodology was developed to assess vitellogenin using high-performance liquid chromatography (HPLC). Exposure to both 17α-ethynylestradiol (50 ng/L) and 4-nonylphenol (100 μg/L) produced increases in vitellogenin for females, whereas males exhibited increases in vitellogenin when exposed to 50 ng/L 17α-ethynylestradiol only. Females exhibited greater vitellogenin responses than males at 50 ng/L 17α-ethynylestradiol only. Histological examination of gonads revealed a number of individuals exhibiting intersex (ovotestis) in 50 ng/L 17α-ethynylestradiol exposures. Male individuals in 1 μg/L and 100 μg/L 4-nonylphenol exposures and 5 ng/L 17α-ethynylestradiol were at earlier stages of spermatogenic development than corresponding controls. © 2008 Elsevier B.V. All rights reserved.
Details
- Title
- Effects of 4-nonylphenol and 17α-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata: Vitellogenin induction and gonadal development
- Authors
- M N Andrew (Author) - University of NewcastleR H Dunstan (Author) - University of NewcastleWayne A O'Connor (Author) - Port Stephens Research CentreL Van Zwieten (Author) - New South Wales Department of Primary IndustriesB Nixon (Author) - University of NewcastleG R MacFarlane (Author) - University of Newcastle
- Publication details
- Aquatic Toxicology, Vol.88(1), pp.39-47
- Publisher
- Elsevier BV
- Date published
- 2008
- DOI
- 10.1016/j.aquatox.2008.03.003
- ISSN
- 0166-445X
- Organisation Unit
- University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
- Language
- English
- Record Identifier
- 99450424702621
- Output Type
- Journal article
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- Domestic collaboration
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- Marine & Freshwater Biology
- Toxicology
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Source: InCites