Effective treatment of squamous cell carcinomas with ingenol mebutate gel in immunologically intact SKH1 mice

S J Cozzi; T T Le; Steven Ogbourne; C James; A Suhrbier

doi:10.1007/s00403-012-1270-0

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Effective treatment of squamous cell carcinomas with ingenol mebutate gel in immunologically intact SKH1 mice

Journal article

Open access

Peer reviewed

Effective treatment of squamous cell carcinomas with ingenol mebutate gel in immunologically intact SKH1 mice

S J Cozzi, T T Le, Steven Ogbourne, C James and A Suhrbier

Archives of Dermatological Research, Vol.305(1), pp.79-83

2013

DOI: https://doi.org/10.1007/s00403-012-1270-0

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Abstract

ingenol mebutate

squamous cell carcinoma

mouse model

Ingenol mebutate has recently been approved by the Federal Drug Administration (USA) as a topical treatment for actinic keratoses. Herein, we describe the efficacy of ingenol mebutate for the topical treatment of squamous cell carcinoma (SCC) using a wild-type mouse model (SKH1) and the UV-induced mouse SCC cell line, T7. Daily treatment for 2 days with 0.25 % ingenol mebutate gel produced a cure rate of 70 %, with 0 % for placebo gel. Electron microscopy revealed swelling of cancer cell mitochondria within 1 h, with disruption of the inner mitochondrial membranes evident at 6 h post treatment. Primary necrosis of cancer cells was clearly evident by 24 h. Treatment was associated with local haemorrhage and a prodigious neutrophil infiltrate, with anti-T7 antibodies also detected. This is the first report of the successful treatment of SCC tumours with ingenol mebutate gel in wild-type mice, and supports the view that ingenol mebutate induces primary necrosis and activates the immune system.

Details

Title: Effective treatment of squamous cell carcinomas with ingenol mebutate gel in immunologically intact SKH1 mice
Authors: S J Cozzi (Author) - Queensland Institute of Medical Research
T T Le (Author) - Queensland Institute of Medical Research
Steven Ogbourne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
C James (Author) - Queensland Institute of Medical Research
A Suhrbier (Author) - Griffith University
Publication details: Archives of Dermatological Research, Vol.305(1), pp.79-83
Publisher: Springer
Date published: 2013
DOI: 10.1007/s00403-012-1270-0
ISSN: 0340-3696
Copyright note: Copyright © The Author(s) 2012. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450151902621
Output Type: Journal article

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