Journal article
Effect of the novel wound healing agent, OPAL A on leukotriene B4 production in human neutrophils and 5-lipoxygenase activity
Wound Practice & Research, Vol.19(4), pp.200-203
2011
Abstract
OPAL A is a papaya pulp that is heated and alkalised with bicarbonate (the OPAL process) and is undergoing clinical trials for treatment of chronic wounds. The aim of this study was to investigate possible inhibitory effects of OPAL A and a non-alkalised papaya filtrate on the 5-lipoxygenase signalling pathway. Human isolated neutrophils were incubated with or without OPALA, non-alkalised papaya or sodium bicarbonate and then exposed to the calcium ionophore, ionomycin to stimulate leukotriene B4 (LTB4) production. The production of LTB4 was inhibited in a dose-dependent manner by all three treatments. The effect of these treatments on 5-lipoxygenase activity, the enzyme involved in the production of precursors of LTB4 was investigated using a cell-free assay. 5-Lipoxygenase activity was inhibited by OPAL A and non-alkalised papaya, but not bicarbonate. Column chromatography was used to show that the active components within OPAL A were non-proteinaceous polar compounds. The inhibitory effects of OPAL A and a non-alkalised papaya filtrate on 5-lipoxygenase activity and LTB4 production suggest a possible anti-inflammatory mode of action.
Details
- Title
- Effect of the novel wound healing agent, OPAL A on leukotriene B4 production in human neutrophils and 5-lipoxygenase activity
- Authors
- Fraser D Russell (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationTanja Windegger (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationKarina D Hamilton (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationNorman W H Cheetham (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
- Publication details
- Wound Practice & Research, Vol.19(4), pp.200-203
- Publisher
- Cambridge Publishing
- Date published
- 2011
- ISSN
- 1837-6304; 1323-2495
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449841702621
- Output Type
- Journal article
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