Journal article
Effect of sodium selenite-enriched reperfusion solutions on rat cardiac ischemia reperfusion injury
Biological Trace Element Research, Vol.114(1-3), pp.197-206
2006
PMID: 17206002
Abstract
Cardiac surgery often generates oxidative stress leading to ischemia reperfusion injury (I-R). Antioxidants have been shown to prevent this injury and have been added to cardioplegic solutions to assist in recovery. In this study, we tested the effectiveness of sodium selenite in protecting against ischemia reperfusion injury and investigated the mechanisms behind this protection. Hearts from male Wistar rats were subjected to ischemia reperfusion using the Langendorf model. Krebs-Henseleit perfusion solutions were supplemented with 0, 0.1, 0.5, 1.0, and 10 microM sodium selenite. Hearts were perfused for 30 min and then subjected to 22.5 min of global ischemia followed by 45 min reperfusion. Heart rate, ischemic contracture, end diastolic pressure, and developed ventricular pressure were monitored. At the completion of the experiment, hearts were homogenized and tissue extracts were assayed for glutathione peroxidase (GSH-Px) and thioredoxin reductase (Thx-Red) activity. Sodium selenite, at a concentration of 0.5 microM, demonstrated a protective effect on the recovery of cardiac function following I-R, as evidenced by a lower end diastolic pressure and enhanced recovery of rate pressure product. There was no beneficial effect observed in hearts perfused with 0.1 microM sodium selenite-supplemented buffer, whereas poorer functional recovery was observed in hearts perfused with 10 microM sodium selenite-supplemented buffer. The beneficial effect of sodium selenite was not mediated through increased activity of GSH-Px or Thx-Red. This study demonstrates that the addition of sodium selenite to reperfusion solutions, at an optimal concentration of 0.5 microM, assists in cardiac recovery following ischemia reperfusion.
Details
- Title
- Effect of sodium selenite-enriched reperfusion solutions on rat cardiac ischemia reperfusion injury
- Authors
- Robyn Lymbury (Author) - Griffith UniversityKylie Venardos (Author) - Griffith UniversityAnthony V Perkins (Corresponding Author) - Griffith University
- Publication details
- Biological Trace Element Research, Vol.114(1-3), pp.197-206
- Publisher
- Springer
- DOI
- 10.1385/BTER:114:1:197
- ISSN
- 1559-0720
- PMID
- 17206002
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy; School of Health
- Language
- English
- Record Identifier
- 99685293202621
- Output Type
- Journal article
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- Biochemistry & Molecular Biology
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Source: InCites