Effect of serial-day exposure to nitrogen dioxide on airway and blood leukocytes and lymphocyte subsets

Colin Solomon; D L Christian; L L Chen; B S Welch; M T Kleinman; E Dunham; D J Erle; J Balmes

doi:10.1034/j.1399-3003.2000.15e19.x

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Effect of serial-day exposure to nitrogen dioxide on airway and blood leukocytes and lymphocyte subsets

Journal article

Peer reviewed

Effect of serial-day exposure to nitrogen dioxide on airway and blood leukocytes and lymphocyte subsets

Colin Solomon, D L Christian, L L Chen, B S Welch, M T Kleinman, E Dunham, D J Erle and J Balmes

European Respiratory Journal, Vol.15(5), pp.922-928

2000

DOI: https://doi.org/10.1034/j.1399-3003.2000.15e19.x

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Abstract

Medical and Health Sciences

inflammation

leukocytes

lymphocytes

Nitrogen dioxide (NO2) is a free radical-producing oxidant gas. Inhalation of NO2 could cause airway inflammation, and decrease immune function. This experiment tested the hypothesis that exposure to NO2 would: 1) increase leukocytes in bronchoalveolar lavage (BAL); and 2) change the distribution of lymphocyte subsets and activation in BAL and peripheral blood (PB). Using a counter-balanced, repeated-measures design, 15 healthy volunteers were exposed to filtered air (FA) or 2.0 parts per million NO2 for 4 h × day(-1) (4 × 30 min of exercise), for three consecutive days. Bronchoscopy was performed 18 h following each exposure set, and PB was drawn pre-exposure and pre-bronchoscopy. Flow cytometry was used to enumerate lymphocyte subsets and activation makers in BAL and PB. In the bronchial fraction, there was an increase in the percentage of neutrophils following NO2 exposure compared to FA (median (interquartile range): 10.6 (4.8-17.2)% versus 5.3 (2.5-8.3)%; p=0.005). In the BAL, there was a decrease in the percentage of T-helper cells following NO2 exposure compared to FA (55.9 (40.8-62.7)% versus 61.6 (52.6-65.2)%; p=0.022). For PB, there were no between-condition differences in any leukocyte or lymphocyte subsets, or activation. In conclusion exposure to nitrogen dioxide results in bronchial inflammation and a minimal change in bronchoalveolar lavage T-helper cells, and no changes in peripheral blood cells.

Details

Title: Effect of serial-day exposure to nitrogen dioxide on airway and blood leukocytes and lymphocyte subsets
Authors: Colin Solomon (Author) - University of California, United States
D L Christian (Author) - University of California, United States
L L Chen (Author) - University of California, United States
B S Welch (Author) - University of California, United States
M T Kleinman (Author) - University of California, United States
E Dunham (Author) - University of California, United States
D J Erle (Author) - University of California, United States
J Balmes (Author) - University of California, United States
Publication details: European Respiratory Journal, Vol.15(5), pp.922-928
Publisher: European Respiratory Society
Date published: 2000
DOI: 10.1034/j.1399-3003.2000.15e19.x
ISSN: 0903-1936
Organisation Unit: Centre for Human Factors and Systems Science; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health - Sports & Exercise Science; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450283402621
Output Type: Journal article

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