Journal article
Effect of Iodine and Selenium on Proliferation, Viability, and Oxidative Stress in HTR-8/SVneo Placental Cells
Biological Trace Element Research, Vol.199(4), pp.1332-1344
2021
PMID: 32623623
Abstract
Adequate maternal micronutrition is vital for placental formation, fetal growth, and development. Oxidative stress adversely affects placental development and function and an association between deficient placental development, oxidative stress, and micronutrient deficiency has been observed. Selenium and iodine are two essential micronutrients with antioxidant properties. Epidemiological studies have shown that poor micronutrient status in pregnant women is associated with a higher incidence of pregnancy complications. The aim of this study was to determine how selenium, iodine, and their combination impact oxidative stress in placental trophoblast cells. HTR8/SVneo extravillous trophoblasts were supplemented with a concentration range of organic and inorganic selenium, potassium iodide, or their combination for 24 h. Oxidative stress was then induced by treating cells with menadione or H(2)O(2)for 24 h. Cell viability and lipid peroxidation as the biomarker of oxidative stress were assessed at 48 h. Both menadione and H(2)O(2)reduced cell viability and increased lipid peroxidation (P < 0.05). Greater cell viability was found in selenium-supplemented cells when compared with vehicle treated cells (P < 0.05). Selenium and iodine supplementation separately or together were associated with lower lipid peroxidation compared with vehicle control (P < 0.05). Supplementation with the combination of selenium and iodine resulted in a greater reduction in lipid peroxidation compared with selenium or iodine alone (P < 0.05). Oxidative stress negatively impacts trophoblast cell survival and cellular integrity. Selenium and iodine protect placental trophoblasts against oxidative stress. Further research is warranted to investigate the molecular mechanisms by which selenium and iodine act in the human placenta.
Details
- Title
- Effect of Iodine and Selenium on Proliferation, Viability, and Oxidative Stress in HTR-8/SVneo Placental Cells
- Authors
- Nahal Habibi (Author) - University of AdelaideTanja Jankovic-Karasoulos (Author) - University of AdelaideShalem Yiner-Lee Leemaqz (Author) - University of AdelaideMaxime Francois (Author) - CSIRO Health and BiosecurityShao Jia Zhou (Author) - University of AdelaideWayne R. Leifert (Author) - CSIRO Health and BiosecurityAnthony Perkins (Author) - Griffith UniversityClaire T. Roberts (Author) - University of AdelaideTina Bianco-Miotto (Author) - University of Adelaide
- Publication details
- Biological Trace Element Research, Vol.199(4), pp.1332-1344
- Publisher
- Springer
- DOI
- 10.1007/s12011-020-02277-7
- ISSN
- 1559-0720
- PMID
- 32623623
- Grant note
- Flinders University Matthew Flinders Fellowship GNT1161079 / National Health and Medical Research Council (NHMRC); National Health and Medical Research Council (NHMRC) of Australia GNT1174971 / NHMRC Investigator Grant; National Health and Medical Research Council (NHMRC) of Australia
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy; School of Health
- Language
- English
- Record Identifier
- 99685196202621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Endocrinology & Metabolism
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