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Published VersionCC BY V4.0, Open Access
Journal article
Effect of Australian propolis from stingless bees (Tetragonula carbonaria) on pre-contracted human and porcine isolated arteries
PLoS One, Vol.8(11), e81297
2013
Abstract
Bee propolis is a mixture of plant resins and bee secretions. While bioactivity of honeybee propolis has been reported previously, information is limited on propolis from Australian stingless bees (Tetragonula carbonaria). The aim of this study was to investigate possible vasomodulatory effects of propolis in KCl-precontracted porcine coronary arteries using an ex vivo tissue bath assay. Polar extracts of propolis produced a dose-dependent relaxant response (EC50=44.7±7.0 μg/ml), which was unaffected by endothelial denudation, suggesting a direct effect on smooth muscle. Propolis markedly attenuated a contractile response to Ca2+ in vessels that were depolarised with 60 mM KCl, in Ca2+-free Krebs solution. Propolis (160 μg/ml) reduced vascular tone in KCl pre-contracted vessels to nearbaseline levels over 90 min, and this effect was partially reversible with 6h washout. Some loss in membrane integrity, but no loss in mitochondrial function was detected after 90 min exposure of human cultured umbilical vein endothelial cells to 160 μg/ml propolis. We conclude that Australian stingless bee (T. carbonaria) propolis relaxes porcine coronary artery in an endothelial-independent manner that involves inhibition of voltage-gated Ca2+ channels. This effect is partially and slowly reversible upon washout. Further studies are required to determine the therapeutic potential of Australian stingless bee propolis for conditions in which vascular supply is compromised.
Details
- Title
- Effect of Australian propolis from stingless bees (Tetragonula carbonaria) on pre-contracted human and porcine isolated arteries
- Authors
- Carmelina F Massaro (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringPeter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringHelen M Wallace (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringVianne Nsengiyumva (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringLorraine Narokai (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringFraser D Russell (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- PLoS One, Vol.8(11), e81297
- Publisher
- Public Library of Science
- Date published
- 2013
- DOI
- 10.1371/journal.pone.0081297
- ISSN
- 1932-6203
- Copyright note
- Copyright © 2013 Massaro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449208002621
- Output Type
- Journal article
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