ERK1/2 activation modulates pyocyanin-induced toxicity in A549 respiratory epithelial cells

Amanda Forbes; Andrew K. Davey; Anthony V. Perkins; Gary D. Grant; Amelia J. McFarland; Catherine M. McDermott; Shailendra Anoopkumar-Dukie

doi:10.1016/j.cbi.2013.11.016

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ERK1/2 activation modulates pyocyanin-induced toxicity in A549 respiratory epithelial cells

Journal article

Peer reviewed

ERK1/2 activation modulates pyocyanin-induced toxicity in A549 respiratory epithelial cells

Amanda Forbes, Andrew K. Davey, Anthony V. Perkins, Gary D. Grant, Amelia J. McFarland, Catherine M. McDermott and Shailendra Anoopkumar-Dukie

Chemico-Biological Interactions, Vol.208(1), pp.58-63

2014

DOI: https://doi.org/10.1016/j.cbi.2013.11.016

PMID: 24316274

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Abstract

A549 cells

Extracellular signal-regulated kinase (ERK)

Mitogen-activated protein kinases (MAPK)

Oxidative stress

Pyocyanin

Pyocyanin (PCN), a virulence factor produced by Pseudomonas aeruginosa, has many damaging effects on mammalian cells. Several lines of evidence suggest that this damage is primarily mediated by its ability to generate oxidative stress. However mechanisms underlying PCN-induced oxidative injury remain unclear. Although oxidative stress and subsequent MAPK signaling has been shown to modulate cell death in other models, its role in PCN-induced cytotoxicity remains unknown. Therefore the aim of this study was to investigate the role of redox-sensitive MAPK in PCN-induced toxicity in A549 cells. Here we show that PCN (50 μM) rapidly increased ERK1/2 phosphorylation after 5 min. Pre-treatment of A549 cells with the MEK1/2 inhibitor U0126 (10 μM) decreased PCN-induced ERK1/2 phosphorylation and protected cells against apoptosis and cell injury suggesting a role for ERK signalling. In contrast, JNK and p38 MAPK phosphorylation remained unchanged following exposure to PCN and pretreatment with either the JNK or p38 MAPK inhibitors (10 μM SP600125 and 10 μM SB203580, respectively) did not afford protection against PCN toxicity. This would suggest that PCN-induced cytotoxicity appears to occur independently of JNK and p38 MAPK signaling pathways. Finally, although we confirm that oxidative stress contributes to PCN-induced toxicity, our data suggest the contribution of oxidative stress is independent of ERK1/2 signaling. These findings may provide insight for novel targeted therapies to reduce PCN-mediated lung injury in patients with chronic P. aeruginosa respiratory infections.

Details

Title: ERK1/2 activation modulates pyocyanin-induced toxicity in A549 respiratory epithelial cells
Authors: Amanda Forbes (Author) - Griffith University
Andrew K. Davey (Author) - Griffith University
Anthony V. Perkins (Author) - Griffith University
Gary D. Grant (Author) - Griffith University
Amelia J. McFarland (Author) - Griffith University
Catherine M. McDermott (Author) - Bond University
Shailendra Anoopkumar-Dukie (Corresponding Author) - Griffith University
Publication details: Chemico-Biological Interactions, Vol.208(1), pp.58-63
Publisher: Elsevier Ireland Ltd
DOI: 10.1016/j.cbi.2013.11.016
ISSN: 1872-7786
PMID: 24316274
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy; School of Health
Language: English
Record Identifier: 99685296402621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology

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