Journal article
Down-regulation of the expression of CCAAT/enhancer binding protein alpha gene in cervical squamous cell carcinoma
BMC Cancer, Vol.14(1), 417
2014
Abstract
Background: Cervical carcinoma is the second most common cancer and is an important cause of death in women worldwide. CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular differentiation and proliferation in a variety of tissues. However, the role of C/EBPalpha gene in cervical cancer is still not clear. Methods We investigated the expression of C/EBPalpha gene in cervical squamous cell carcinoma. C/EBPalpha mRNA level was measured by real-time quantitative RT-PCR in cervical cancer tissues and their adjacent normal tissues. C/EBPalpha protein level was measured by immunohistochemistry. Methylation in the promoter of C/EBPalpha gene was detected by MALDI TOF MassARRAY. We transfected HeLa cells with C/EBPalpha expression vector. C/EBPalpha expression in HeLa cells was examined and HeLa cell proliferation was measured by MTT assay and HeLa cells migration was analyzed by matrigel-coated transwell migration assays. Results There were significant difference in C/EBPalpha protein expression between chronic cervicitis and cervical carcinoma (P less than 0.001). CEBPalpha mRNA level was significantly lower in cervical cancer tissues than in normal cervical tissues (P less than 0.01). Methylation of the promoter of CEBPalpha gene in CpG 5, CpG-14.15, CpG-19.20 were significantly higher in cervical cancer than in normal cervical tissues (P less than 0.05, P less than 0.01, P less than 0.05, respectively). CEBPalpha pcDNA3.1 construct transfected into HeLa cells inhibited cell proliferation and decreased cell migration. Conclusions Our results indicate that reduced C/EBPalpha gene expression may play a role in the development of cervical squamous cell carcinoma.
Details
- Title
- Down-regulation of the expression of CCAAT/enhancer binding protein alpha gene in cervical squamous cell carcinoma
- Authors
- Zemin Pan (Author) - Shihezi University, ChinaWeinan Zheng (Author) - Shihezi University, ChinaJinli Zhang (Author) - Shihezi University, ChinaRui Gao (Author) - Shihezi University, ChinaDongmei Li (Author) - Shihezi University, ChinaXiaoqing Guo (Author) - Shihezi University, ChinaHu Han (Author) - Shihezi University, ChinaFeng Li (Author) - Shihezi University, ChinaShen Qu (Author) - Huazhong University of Science and Technology, ChinaRenfu Shao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- BMC Cancer, Vol.14(1), 417; 16
- Publisher
- BioMed Central Ltd.
- Date published
- 2014
- DOI
- 10.1186/1471-2407-14-417
- ISSN
- 1471-2407
- Copyright note
- Copyright © 2014 Pan et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448954002621
- Output Type
- Journal article
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