Down-regulation of IFITM1 and its growth inhibitory role in cervical squamous cell carcinoma

Weinan Zheng; Zhimin Zhao; Xinan Yi; Qiangqiang Zuo; Hongtao Li; Xiaoqing Guo; Dongmei Li; Hongchang He; Zemin Pan; Peiwen Fan; Feng Li; Yanhong Liao; Renfu Shao

doi:10.1186/s12935-017-0456-0

Back

Down-regulation of IFITM1 and its growth inhibitory role in cervical squamous cell carcinoma

Journal article

Open access

Peer reviewed

Down-regulation of IFITM1 and its growth inhibitory role in cervical squamous cell carcinoma

Weinan Zheng, Zhimin Zhao, Xinan Yi, Qiangqiang Zuo, Hongtao Li, Xiaoqing Guo, Dongmei Li, Hongchang He, Zemin Pan, Peiwen Fan, …

Cancer Cell International, Vol.17, 88

2017

DOI: https://doi.org/10.1186/s12935-017-0456-0

Files and links (2)

pdf

PDF - Published Version (Open Access)1.74 MBDownload View

Published VersionPDF - Published Version (Open Access)CC BY V4.0, Open Access

url

https://doi.org/10.1186/s12935-017-0456-0View

Published Version

Abstract

IFITM1 gene

cell proliferation

migration and invasion

cervical squamous cell carcinoma

Background: Cervical cancer is a major cause of death in women worldwide. Interferon-induced transmembrane protein 1 (IFITM1) is involved in antivirus defense, cell adhesion, and carcinogenesis in diferent tissues. However, the role of IFITM1 gene in cervical squamous cell cancer is unclear. Methods: To explore the role of IFITM1 in carcinogenesis of cervical cancer, we investigated the expression of IFITM1 gene in cervical squamous cell carcinoma. IFITM1 mRNA level was measured by real-time quantitative RT-PCR in cervical cancer tissues and their adjacent normal tissues. IFITM1 protein level was measured by immunohistochemistry. Methylation in the IFITM1 gene promoter was detected by methylation-specifc PCR. We then transfected HeLa cells with IFITM1 expression vector or control vector. IFITM1 expression was examined; cell migration and invasion were analyzed by wound healing assay and matrigel-coated transwell migration assays, respectively. HeLa cell proliferation was measured by cell counting kit-8 assay and cell cycle analysis. Cell apoptosis was analyzed by Annexin V/propidium iodide double staining assay. Results: The diference in IFITM1 protein expression between samples from chronic cervicitis and cervical carcinoma was statistically signifcant (P < 0.01). Ki-67 and PCNA protein expression levels were signifcantly higher in cervical cancer tissues than in their corresponding cervicitis tissues (P < 0.05 and P < 0.001, respectively). IFITM1 mRNA level was signifcantly lower in cervical cancer tissues than in normal cervical tissues (P < 0.05). Methylation of the IFITM1 gene promoter was signifcantly higher in cervical cancer than in normal cervical tissues (P < 0.05). Transfection of the IFITM1 pcDNA3.1 construct decreased cell migration and invasion of HeLa cells, inhibited cell proliferation, and increased cell apoptosis. Conclusion: IFITM1 gene expression may reduce the proliferation, migration, and invasion of cervical squamous cancer cells.

Details

Title: Down-regulation of IFITM1 and its growth inhibitory role in cervical squamous cell carcinoma
Authors: Weinan Zheng (Author) - Huazhong University of Science and Technology, China
Zhimin Zhao (Author) - Huazhong University of Science and Technology, China
Xinan Yi (Author) - Hainan Medical University, China
Qiangqiang Zuo (Author) - Shihezi University, China
Hongtao Li (Author) - Shihezi University, China
Xiaoqing Guo (Author) - Shihezi University, China
Dongmei Li (Author) - Shihezi University, China
Hongchang He (Author) - Shihezi University, China
Zemin Pan (Author) - Huazhong University of Science and Technology, China
Peiwen Fan (Author) - Shihezi University, China
Feng Li (Author) - Shihezi University, China
Yanhong Liao (Author) - Huazhong University of Science and Technology, China
Renfu Shao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Cancer Cell International, Vol.17, 88; 12
Publisher: BioMed Central Ltd.
Date published: 2017
DOI: 10.1186/s12935-017-0456-0
ISSN: 1475-2867
Copyright note: Copyright © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451156902621
Output Type: Journal article

Metrics

45 File views/ downloads

706 Record Views

11 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Oncology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites