Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla—Towards Finding the Missing Link in Achelata

Thomas M Banks; Tianfang Wang; Quinn P Fitzgibbon; Gregory G Smith; Tomer Ventura

doi:10.3390/ijms21186967

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Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla—Towards Finding the Missing Link in Achelata

Journal article

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Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla—Towards Finding the Missing Link in Achelata

Thomas M Banks, Tianfang Wang, Quinn P Fitzgibbon, Gregory G Smith and Tomer Ventura

International Journal of Molecular Sciences, Vol.21(18), pp.1-20

2020

DOI: https://doi.org/10.3390/ijms21186967

PMCID: PMC7554946

PMID: 32971953

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Abstract

serum dsRNA binding proteins

gene silencing mechanism

systemic RNAi

decapod crustaceans

dsRNA transport

RNA interference (RNAi) has become a widely utilized method for studying gene function, yet despite this many of the mechanisms surrounding RNAi remain elusive. The core RNAi machinery is relatively well understood, however many of the systemic mechanisms, particularly double-stranded RNA (dsRNA) transport, are not. Here, we demonstrate that dsRNA binding proteins in the serum contribute to systemic RNAi and may be the limiting factor in RNAi capacity for species such as spiny lobsters, where gene silencing is not functional. Incubating sera from a variety of species across phyla with dsRNA led to a gel mobility shift in species in which systemic RNAi has been observed, with this response being absent in species in which systemic RNAi has never been observed. Proteomic analysis suggested lipoproteins may be responsible for this phenomenon and may transport dsRNA to spread the RNAi signal systemically. Following this, we identified the same gel shift in the slipper lobster Thenus australiensis and subsequently silenced the insulin androgenic gland hormone, marking the first time RNAi has been performed in any lobster species. These results pave the way for inducing RNAi in spiny lobsters and for a better understanding of the mechanisms of systemic RNAi in Crustacea, as well as across phyla.

Details

Title: Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla—Towards Finding the Missing Link in Achelata
Authors: Thomas M Banks (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Tianfang Wang (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Quinn P Fitzgibbon (Author) - University of Tasmania
Gregory G Smith (Author) - University of Tasmania
Tomer Ventura (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Publication details: International Journal of Molecular Sciences, Vol.21(18), pp.1-20
Publisher: MDPI AG
Date published: 2020
DOI: 10.3390/ijms21186967
ISSN: 1422-0067
PMID: 32971953; PMC7554946
Copyright note: Copyright (c) 2020 The Authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Grants: The ARC Research Hub for Sustainable Lobster Aquaculture, IH190100014, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Science and Engineering - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99482308402621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary

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