Dopamine-mediated striatal activity and function is enhanced in GlyRα2 knockout animals

Jens Devoght; Joris Comhair; Giovanni Morelli; Jean-Michel Rigo; Rudi D'Hooge; Chadi Touma; Rupert Palme; Ilse Dewachter; Martin vandeVen; Robert J. Harvey; Serge Schiffmann; Elisabeth Piccart; Bert Brône

doi:10.1016/j.isci.2023.107400

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Dopamine-mediated striatal activity and function is enhanced in GlyRα2 knockout animals

Journal article

Open access

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Dopamine-mediated striatal activity and function is enhanced in GlyRα2 knockout animals

Jens Devoght, Joris Comhair, Giovanni Morelli, Jean-Michel Rigo, Rudi D'Hooge, Chadi Touma, Rupert Palme, Ilse Dewachter, Martin vandeVen, Robert J. Harvey, …

iScience, Vol.26(8), pp.1-21

2023

DOI: https://doi.org/10.1016/j.isci.2023.107400

PMCID: PMC10404725

PMID: 37554441

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Abstract

neuroscience

behaviorial neuroscience

The glycine receptor alpha 2 (GlyRα2) is a ligand-gated ion channel which upon activation induces a chloride conductance. Here, we investigated the role of GlyRα2 in dopamine-stimulated striatal cell activity and behavior. We show that depletion of GlyRα2 enhances dopamine-induced increases in the activity of putative dopamine D1 receptor-expressing striatal projection neurons, but does not alter midbrain dopamine neuron activity. We next show that the locomotor response to d-amphetamine is enhanced in GlyRα2 knockout animals, and that this increase correlates with c-fos expression in the dorsal striatum. 3-D modeling revealed an increase in the neuronal ensemble size in the striatum in response to D-amphetamine in GlyRα2 KO mice. Finally, we show enhanced appetitive conditioning in GlyRα2 KO animals that is likely due to increased motivation, but not changes in associative learning or hedonic response. Taken together, we show that GlyRα2 is an important regulator of dopamine-stimulated striatal activity and function.

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Title: Dopamine-mediated striatal activity and function is enhanced in GlyRα2 knockout animals
Authors: Jens Devoght (Author) - University of Hasselt
Joris Comhair (Author) - University of Hasselt
Giovanni Morelli (Author) - Italian Institute of Technology
Jean-Michel Rigo (Author) - University of Hasselt
Rudi D'Hooge (Author) - KU Leuven
Chadi Touma (Author) - University of Osnabrück
Rupert Palme (Author) - University of Veterinary Medicine Vienna
Ilse Dewachter (Author) - University of Hasselt
Martin vandeVen (Author) - University of Hasselt
Robert J. Harvey (Author) - University of the Sunshine Coast, Queensland, School of Health
Serge Schiffmann (Author) - Université Libre de Bruxelles
Elisabeth Piccart (Author) - University of Hasselt
Bert Brône (Corresponding Author) - University of Hasselt
Publication details: iScience, Vol.26(8), pp.1-21
Publisher: Cell Press
Date published: 2023
DOI: 10.1016/j.isci.2023.107400
ISSN: 2589-0042
PMID: 37554441; PMC10404725
Copyright note: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: School of Health
Language: English
Record Identifier: 99741896202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Neurosciences

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