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Diversity of glycine receptors in the mouse retina: Localization of the α2 subunit
Journal article   Peer reviewed

Diversity of glycine receptors in the mouse retina: Localization of the α2 subunit

S Haverkamp, U Müller, H U Zeilhofer, Robert J Harvey and H Wässle
Journal of Comparative Neurology, Vol.477(4), pp.399-411
2004
DOI: https://doi.org/10.1002/cne.20267
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https://doi.org/10.1002/cne.20267View
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Abstract

mouse retina glycine receptors α1 subunit α2 subunit α3 subunit
γ-Aminobutyric acid (GABA) and glycine are the major inhibitory neurotransmitters in the retina, glycine being produced in approximately half of all amacrine cells. Whereas retinal cell types expressing the glycine receptor (GlyR) α1 and α3 subunits have been mapped, the role of the α2 subunit in retinal circuitry remains unclear. By using immunocytochemistry, we localized the α2 subunit in the inner plexiform layer (IPL) in brightly fluorescent puncta, which represent postsynaptically clustered GlyRs. This was shown by doubly labeling sections for GlyR α2 and bassoon (a presynaptic marker) or gephyrin (a postsynaptic marker). Synapses containing GlyR α2 were rarely found on ganglion cell dendrites but were observed on bipolar cell axon terminals and on amacrine cell processes. Recently, an amacrine cell type has been described that is immunopositive for glycine and for the vesicular glutamate transporter vGluT3. The processes of this cell type were presynaptic to GlyR α2 puncta, suggesting that vGluT3 amacrine cells release glycine. Double labeling of sections for GlyR α1 and GlyR α2 subunits showed that they are clustered at different synapses. In sections doubly labeled for GlyR α2 and GlyR α3, approximately one-third of the puncta were colocalized. The most abundant GlyR subtype in retina contains α3 subunits, followed by those containing GlyR α2 and GlyR α1 subunits. © 2004 Wiley-Liss, Inc.

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