Diversity of glycine receptors in the mouse retina: Localization of the α3 subunit

S Haverkamp; U Müller; K Harvey; Robert J Harvey; H Betz; H Wässle

doi:10.1002/cne.10852

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Diversity of glycine receptors in the mouse retina: Localization of the α3 subunit

Journal article

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Diversity of glycine receptors in the mouse retina: Localization of the α3 subunit

S Haverkamp, U Müller, K Harvey, Robert J Harvey, H Betz and H Wässle

Journal of Comparative Neurology, Vol.465(4), pp.524-539

2003

DOI: https://doi.org/10.1002/cne.10852

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Abstract

mouse retina

glycine receptors

α3 subunit

GlyRα3 k.o. mouse

GlyRα3antiserum

Glycine receptors (GlyRs) and their role in retinal circuitry were analyzed immunocytochemically in wild-type and GlyR α3 subunit-deficient (Glra3-/-) mouse retinae. GlyRs are localized in the inner plexiform layer in brightly fluorescent puncta, which are likely to represent postsynaptically clustered GlyRs. Approximately one third of the clusters were found to contain the α1 subunit, and half possessed the α3 subunit. However, these two GlyR isoforms were localized at different glycinergic synapses. In the Glra3-/- mouse, α3 subunit clusters were completely eliminated, although the total number of GlyR clusters was only slightly reduced. This finding indicates that other GlyR subunits (such as α2 or α4) may have compensated for the loss of the α3 subunit. Characteristic expression patterns of the α1 and α3 subunits within the synaptic circuits of the retina were revealed by double labeling sections for GlyRs and markers that define specific retinal neurons. The α1 subunit mediates signal transfer in the rod pathway between AII amacrine cells and OFF-cone bipolar cells. In contrast, the α3 subunit appears to be predominantly involved with the cone pathways. Thus, expression of different GlyR α subunit genes correlates with anatomically defined connectivities. © 2003 Wiley-Liss, Inc.

Details

Title: Diversity of glycine receptors in the mouse retina: Localization of the α3 subunit
Authors: S Haverkamp (Author) - Max-Planck-Institute for Brain Research, Germany
U Müller (Author) - Max-Planck-Institute for Brain Research, Germany
K Harvey (Author) - University College London, United Kingdom
Robert J Harvey (Author) - University College London, United Kingdom
H Betz (Author) - Max-Planck-Institute for Brain Research, Germany
H Wässle (Author) - Max-Planck-Institute for Brain Research, Germany
Publication details: Journal of Comparative Neurology, Vol.465(4), pp.524-539
Publisher: John Wiley & Sons Inc.
Date published: 2003
DOI: 10.1002/cne.10852
ISSN: 0021-9967
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450327402621
Output Type: Journal article

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Web Of Science research areas: Neurosciences; Zoology

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