Journal article
Diversity analysis and genotyping in Pisum with sequence tagged microsatellite site (STMS) primers
Euphytica, Vol.124(3), pp.397-405
2002
Abstract
Pisum sativum specific sequence tagged microsatellite site primers were used to amplify genomic profiles from 15 accessions of P. sativum L. that represented the genetic base of the Australian field pea-breeding program and five accessions of the wild related species P. fulvum. The STMS primers were used to assess genetic relationships among the Pisum accessions in two ways. Firstly, to produce RAPD-like multiple banding marker profiles using an adapted RAMS method, for intra- and interspecific diversity analysis. From the 14 flanking primer pairs assessed, 133 markers were obtained. Conservation and reproducibility of markers among individuals within accessions was demonstrated. The largest distance observed among P. sativum accessions was 22% and among P. fulvum accessions was 40%, similar to that revealed with other PCR-based methods. The maximum distance between P. sativum and P. fulvum accessions was 46%. Phylogenetic clustering of P. sativum accessions, using the neighbour joining method and based on simple matching distances, was distinct and distant to P. fulvum. Secondly, PCR with a higher annealing temperature and fluorescent labeling identified simple and allelic loci markers useful for creating a genotype/fingerprint database for P. sativum cultivars. This is the first report to demonstrate the use of Pisum specific STMS sequences for both diversity analysis and genotype identification.
Details
- Title
- Diversity analysis and genotyping in Pisum with sequence tagged microsatellite site (STMS) primers
- Authors
- R Ford (Author) - University of MelbourneK Le Roux (Author) - University of MelbourneCatherine Itman (Author) - University of MelbourneJ B Brouwer (Author) - Department of Natural ResourcesP W J Taylor (Author) - University of Melbourne
- Publication details
- Euphytica, Vol.124(3), pp.397-405
- Publisher
- Springer Netherlands
- Date published
- 2002
- DOI
- 10.1023/A:1015752907108
- ISSN
- 0014-2336
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99449467102621
- Output Type
- Journal article
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- Domestic collaboration
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- Agronomy
- Horticulture
- Plant Sciences
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