Distribution of class 1 integrons among enteropathogenic Escherichia coli

S Najibi; B Bakhshi; S Fallahzad; M R Pourshafie; Mohammad Katouli; M Sattari; M Alebouyeh; M Tajbakhsh

doi:10.1139/W2012-035

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Distribution of class 1 integrons among enteropathogenic Escherichia coli

Journal article

Peer reviewed

Distribution of class 1 integrons among enteropathogenic Escherichia coli

S Najibi, B Bakhshi, S Fallahzad, M R Pourshafie, Mohammad Katouli, M Sattari, M Alebouyeh and M Tajbakhsh

Canadian Journal of Microbiology, Vol.58(5), pp.637-643

2012

DOI: https://doi.org/10.1139/W2012-035

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Abstract

enteropathogenic Escherichia coli

antibiotic resistance

class 1 integrons

The aim of this study was to investigate the incidence of and resistance gene content of class 1 integrons among enteropathogenic Escherichia coli (EPEC) and non-EPEC and to investigate intraspecies genetic diversity of EPEC strains isolated from children with diarrhea in Iran. Twenty-eight EPEC and 16 non-EPEC strains isolated from children with diarrhea were tested for the presence of a class 1 integron associated integrase gene (int1). Sequence analysis was performed to identify the resistance gene content of integrons. Genetic diversity and cluster analysis of EPEC isolates were also investigated using enterobacterial repetitive intergenic concensus - polymerase chain reaction (ERIC-PCR) fingerprinting. Twenty-three (82%) EPEC isolates and 11 (68.7%) non-EPEC isolates harbored the int1 gene specific to the conserved integrase region of class 1 integrons. Sequence analysis revealed the dominance of dfrA and aadA gene cassettes among the isolates of both groups. ERIC-PCR fingerprinting of EPEC isolates revealed a high diversity among these isolates. The widespread distribution of 2 resistance gene families (dfrA and aadA) among both groups of EPEC and non-EPEC isolates indicates the significance of integrons in antibiotic resistance transfer among these bacteria. Furthermore, clonal diversity of EPEC isolates harbouring a class 1 integron also suggests the circulation of these mobile elements among a diverse population of EPEC in this country.

Details

Title: Distribution of class 1 integrons among enteropathogenic Escherichia coli
Authors: S Najibi (Author) - Tarbiat Modares University, Iran
B Bakhshi (Author) - Tarbiat Modares University, Iran
S Fallahzad (Author) - Tarbiat Modares University, Iran
M R Pourshafie (Author) - Pasteur Institute of Iran
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
M Sattari (Author) - Tarbiat Modares University, Iran
M Alebouyeh (Author) - Shaheed Beheshti University of Medical Sciences, Iran
M Tajbakhsh (Author) - Shaheed Beheshti University of Medical Sciences, Iran
Publication details: Canadian Journal of Microbiology, Vol.58(5), pp.637-643
Publisher: N R C Research Press
Date published: 2012
DOI: 10.1139/W2012-035
ISSN: 0008-4166
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449993302621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology

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